PDBe 1ca0

X-ray diffraction
2.1Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Chymotrypsin A chain A Chains: A, F
Molecule details ›
Chains: A, F
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 1-13; Coverage: 5%)
Chymotrypsin A chain B Chains: B, G
Molecule details ›
Chains: B, G
Length: 131 amino acids
Theoretical weight: 13.93 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00766 (Residues: 16-146; Coverage: 54%)
Structure domains: Trypsin-like serine proteases
Chymotrypsin A chain C Chains: C, H
Molecule details ›
Chains: C, H
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00766 (Residues: 149-245; Coverage: 40%)
Structure domains: Trypsin-like serine proteases
Soluble APP-beta Chains: D, I
Molecule details ›
Chains: D, I
Length: 54 amino acids
Theoretical weight: 6.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05067 (Residues: 289-342; Coverage: 7%)
Gene names: A4, AD1, APP
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Factor Xa Inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 70.522Å b: 181.488Å c: 46.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.323
Expression system: Escherichia coli