PDBe 1c3t

Solution NMR

ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURAL SPECIFICITY

Released:
Source organism: Homo sapiens
Primary publication:
Rotamer strain as a determinant of protein structural specificity.
Protein Sci. 8 2598-610 (1999)
PMID: 10631975

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: RESTRAINED MOLECULAR DYNAMICS/SIMULATED ANNEALING USING THE ARIA PROCEDURE OF NILGES (NILGES ET AL., J MOL BIOL, 1997, 269: 408- 422) AND EXPLICIT SWAPPING OF NON- STEREOSPECIFICALLY ASSIGNED METHYLS AND METHYLENES (FOLMER ET AL. J BIOMOL NMR, 1997, 9: 245-258).
Chemical shifts: BMR4663  
Expression system: Escherichia coli BL21