PDBe 1bkd

X-ray diffraction
2.8Å resolution

COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Son of sevenless homolog 1 Chain: S
Molecule details ›
Chain: S
Length: 477 amino acids
Theoretical weight: 56.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07889 (Residues: 568-1044; Coverage: 36%)
Gene name: SOS1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I422
Unit cell:
a: 142.7Å b: 142.7Å c: 207.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.281
Expression system: Escherichia coli BL21(DE3)