PDBe 1bgx

X-ray diffraction
2.3Å resolution

TAQ POLYMERASE IN COMPLEX WITH TP7, AN INHIBITORY FAB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: T
Molecule details ›
Chain: T
Length: 832 amino acids
Theoretical weight: 94.05 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 1-832; Coverage: 100%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
TP7 MAB Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 23.08 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
TP7 MAB Chain: H
Molecule details ›
Chain: H
Length: 213 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P1
Unit cell:
a: 76.6Å b: 89.1Å c: 89.3Å
α: 100.7° β: 115.3° γ: 95.3°
R-values:
R R work R free
0.189 0.189 0.253
Expression systems:
  • Escherichia coli
  • Mus musculus