› 1bf2

X-ray diffraction
2Å resolution

STRUCTURE OF PSEUDOMONAS ISOAMYLASE

Released:
DOI: 10.2210/pdb1bf2/pdb
PDB entry 1bf2 coloured by chain, front view.
PDB entry 1bf2 coloured by chain, side view.
PDB entry 1bf2 coloured by chain, top view.
Source organism: Pseudomonas amyloderamosa
Primary publication:
Three-dimensional structure of Pseudomonas isoamylase at 2.2 A resolution.
Katsuya Y, Mezaki Y, Kubota M, Matsuura Y
J Mol Biol 281 885-97 (1998)
PMID: 9719642

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoamylase Chain: A
Molecule details ›
Chain: A
Length: 750 amino acids
Theoretical weight: 81.14 KDa
Source organism: Pseudomonas amyloderamosa
UniProt:
  • Canonical: P10342 (Residues: 27-776; Coverage: 100%)
Gene name: iam
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 138.9Å b: 152.4Å c: 53.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.214

Quick links

Citations

7 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
6 more

9 mentions without citation

Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus.
Woo et al. (2008)
8 more