PDBe 1bd2

X-ray diffraction
2.5Å resolution

COMPLEX BETWEEN HUMAN T-CELL RECEPTOR B7, VIRAL PEPTIDE (TAX) AND MHC CLASS I MOLECULE HLA-A 0201

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Protein Tax-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell leukemia virus type I
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor beta variable 6-5; T cell receptor beta constant 2 Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: A0A0K0K1A5 (Residues: 20-114; Coverage: 100%)
  • nullnull
Gene names: TCRBC2, TRBC2, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor alpha variable 29/delta variable 5; T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 2-95; Coverage: 66%)
  • Canonical: P04437 (Residues: 28-118; Coverage: 93%)
  • nullnull
Gene names: TCRA, TRAC, TRAV29DV5
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 63.8Å b: 73.3Å c: 217.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.312
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)