PDBe 1b69

Solution NMR

THE SOLUTION STRUCTURE OF TN916 INTEGRASE N-TERMINAL DOMAIN/DNA COMPLEX

Released:
Primary publication:
NMR structure of the Tn916 integrase-DNA complex.
Nat. Struct. Biol. 6 366-73 (1999)
PMID: 10201406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase from transposon Tn916 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.18 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22886 (Residues: 3-71; Coverage: 17%)
Gene name: Int-Tn
Structure domains: Classic Zinc Finger
DNA (5'-D(*GP*AP*AP*TP*TP*TP*AP*CP*TP*AP*CP*TP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*AP*GP*TP*AP*GP*TP*AP*AP*AP*TP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY AND SIMULATED ANNEALING
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided