PDBe 1ao7

X-ray diffraction
2.6Å resolution

COMPLEX BETWEEN HUMAN T-CELL RECEPTOR, VIRAL PEPTIDE (TAX), AND HLA-A 0201

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero decamer
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Protein Tax-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-lymphotropic virus 1
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-cell receptor beta-2 chain C region; T-cell receptor beta variable 6-5 Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: A0A0K0K1A5 (Residues: 20-114; Coverage: 100%)
  • nullnull
Gene names: TCRBC2, TRBC2, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins
T-cell receptor alpha chain C region; T-cell receptor alpha variable 12-2 Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-95; Coverage: 67%)
  • Canonical: A0A075B6T6 (Residues: 23-112; Coverage: 97%)
  • nullnull
Gene names: TCRA, TRAC, TRAV12-2
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 229.3Å b: 49.5Å c: 96Å
α: 90° β: 89.6° γ: 90°
R-values:
R R work R free
0.245 0.245 0.32
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)