PDBe 1a9n

X-ray diffraction
2.38Å resolution

CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
U2 small nuclear ribonucleoprotein A' Chains: A, C
Molecule details ›
Chains: A, C
Length: 176 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P09661 (Residues: 1-176; Coverage: 69%)
Gene name: SNRPA1
Sequence domains: Leucine-rich repeat
Structure domains: Ribonuclease Inhibitor
U2 small nuclear ribonucleoprotein B'' Chains: B, D
Molecule details ›
Chains: B, D
Length: 96 amino acids
Theoretical weight: 11.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P08579 (Residues: 1-96; Coverage: 43%)
Gene name: SNRPB2
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*CP*CP*UP*GP*GP*UP*AP*UP*UP*GP*CP*AP*GP*UP*AP*CP*CP*UP*CP*CP*AP*GP* GP*U)-3') Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 24 nucleotides
Theoretical weight: 7.62 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 98.37Å b: 128.24Å c: 66.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.282 0.328
Expression system: Escherichia coli BL21(DE3)