EMD-9598

Single-particle
11.6 Å
EMD-9598
3D View Gallery
Deposition: 26/07/2018
Map released: 08/08/2018
Last modified: 22/08/2018
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EMD-9598
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Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2

EMD-9598

Single-particle
11.6 Å
EMD-9598
3D View Gallery
Deposition: 26/07/2018
Map released: 08/08/2018
Last modified: 22/08/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 5.8
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 11.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 41289
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 1.4 -
⦨ Initial angle assignment
Type: COMMON LINE
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 88 88 88
Origin 0 0 0
Spacing 88 88 88
Voxel size 2.64 Å 2.64 Å 2.64 Å
Contour list
Primary Level Source
True 0.05 AUTHOR