EMD-9595
Trypsin-cleaved SARS-CoV spike glycoprotein
EMD-9595
Single-particle6.8 Å
Deposition: 26/07/2018
Map released: 08/08/2018
Last modified: 22/08/2018
Buffer
pH: 7.2
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Final
reconstruction
Resolution: 6.8
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 29848
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 29848
⌯ Applied Symmetry
Point group:
C3
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
⦨ Initial angle
assignment
Type:
COMMON LINE
⦩ Final angle assignment
Type:
PROJECTION MATCHING
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 124 | 124 | 124 |
Origin | 0 | 0 | 0 |
Spacing | 124 | 124 | 124 |
Voxel size | 2.54 Å | 2.54 Å | 2.54 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.15 | AUTHOR |