EMDB EMD-9525

Single particle reconstruction
3.4Å resolution

Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution

Map released:
Last modified:
Overview of EMD-9525
Fitted atomic model: 5gmk
Related EM entry by publication: EMD-9526, EMD-9527
Primary publication:
Structure of a yeast catalytic step I spliceosome at 3.4 angstrom resolution
Wan R, Yan C, Bai R, Huang G, Shi Y
Science 353 895-904 (2016)
PMID: 27445308

Function and Biology Details

Sample name: Spliceosomal C Complex
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ZINC ION
Nucleic acids: U5 snRNA, U6 snRNA, U2 snRNA, Intron_BPS, 5'-Exon, 5'-Splicing Site
Proteins: Spliceosomal C Complex, Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor BUD31, Pre-mRNA-splicing factor CWC22, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor CLF1, Protein CWC16, Pre-mRNA-splicing factor CWC25, Pre-mRNA-splicing factor ISY1, Pre-mRNA-splicing factor SYF2, Pre-mRNA-splicing factor SYF1, Pre-mRNA-processing factor 17, Pre-mRNA-processing factor 19, Pre-mRNA-splicing factor SNT309, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, U2 small nuclear ribonucleoprotein B'', U2 small nuclear ribonucleoprotein A'

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)