Electron Microscopy Data Bank

EMD-9524

Single-particle
3.5 Å

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Deposition: 18/08/2016
Map released: 21/09/2016
Last modified: 06/11/2019

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EMD-9524

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Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution

EMD-9524

Single-particle
3.5 Å
EMD-9524

3D View Gallery

Deposition: 18/08/2016
Map released: 21/09/2016
Last modified: 06/11/2019

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Name: Bact spliceosomal complex

Summary:

Complex

Bact spliceosomal complex

Protein

Pre-mRNA-splicing factor 8
Pre-mRNA-splicing helicase BRR2
Pre-mRNA-splicing factor SNU114
Pre-mRNA-splicing factor RSE1
Cold sensitive U2 snRNA suppressor 1
Pre-mRNA-splicing factor PRP11
Pre-mRNA-splicing factor RDS3
RDS3 complex subunit 10
Pre-mRNA-splicing factor PRP46
Pre-mRNA-processing protein 45
Pre-mRNA-splicing factor SLT11
Pre-mRNA-splicing factor CWC2
Pre-mRNA-splicing factor CWC15
Pre-mRNA-splicing factor BUD31
Pre-mRNA leakage protein 1
U2 snRNP component IST3
Pre-mRNA-splicing factor CWC26
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
Pre-mRNA-splicing factor CWC22
Pre-mRNA-splicing factor CWC24
Peptidyl-prolyl isomerase CWC27
Pre-mRNA-splicing factor CEF1
U2 snRNP component HSH155
Pre-mRNA-splicing factor CLF1
Pre-mRNA-splicing factor CWC21
Pre-mRNA-splicing factor SYF1
Protein HSH49
Pre-mRNA-splicing factor SYF2
Pre-mRNA-processing factor 19
Pre-mRNA-splicing factor SNT309
Pre-mRNA-processing factor 17
Small nuclear ribonucleoprotein-associated protein B
Small nuclear ribonucleoprotein E
Small nuclear ribonucleoprotein F
Small nuclear ribonucleoprotein G
Small nuclear ribonucleoprotein Sm D3
Small nuclear ribonucleoprotein Sm D1
Small nuclear ribonucleoprotein Sm D2

Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence
Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence
U2 snRNA
Pre-mRNA
Pre-mRNA

Ligand

GUANOSINE-5'-TRIPHOSPHATE
MAGNESIUM ION
ZINC ION
ADENOSINE-5'-DIPHOSPHATE

Complex [1]

Name: Bact spliceosomal complex

Natural source [1]

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	2 MDa	-

Complex Portal [1]

ID	Name	Overlap
CPX-1651	PRP19-associated complex	0.58

Protein [38]

LEVO

Name: Pre-mRNA-splicing factor 8
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [20]

Biological process:
spliceosomal snRNP assembly mRNA splicing, via spliceosome generation of catalytic spliceosome for second transesterification step mRNA 3'-splice site recognition spliceosomal tri-snRNP complex assembly mRNA 5'-splice site recognition
Molecular function:
mRNA binding metallopeptidase activity isopeptidase activity U6 snRNA binding pre-mRNA intronic binding U5 snRNA binding U2 snRNA binding U1 snRNA binding
Cellular location:
spliceosomal complex cytoplasm U5 snRNP U4/U6 x U5 tri-snRNP complex nucleus catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	265 kDa	-

LEVO

Name: Pre-mRNA-splicing helicase BRR2
EC number: 3.6.4.13
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [11]

Biological process:
mRNA splicing, via spliceosome spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
Molecular function:
ATP binding RNA helicase activity identical protein binding nucleic acid binding ATP hydrolysis activity
Cellular location:
U5 snRNP U4/U6 x U5 tri-snRNP complex spliceosomal complex nucleus

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	246 kDa	-

LEVO

Name: Pre-mRNA-splicing factor SNU114
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [17]

Biological process:
spliceosomal snRNP assembly generation of catalytic spliceosome for first transesterification step spliceosomal tri-snRNP complex assembly mRNA splicing, via spliceosome spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
Molecular function:
translation elongation factor activity GTP binding U5 snRNA binding GTPase activity
Cellular location:
cytosol spliceosomal complex cytoplasm U4/U6 x U5 tri-snRNP complex U2-type catalytic step 2 spliceosome nucleus ribonucleoprotein complex U5 snRNP

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	114 kDa	-

LEVO

Name: Pre-mRNA-splicing factor RSE1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [9]

Biological process:
U2-type prespliceosome assembly spliceosomal complex assembly mRNA splicing, via spliceosome
Molecular function:
U2 snRNA binding
Cellular location:
spliceosomal complex nucleus U2 snRNP U2-type spliceosomal complex U2-type prespliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	153 kDa	-

LEVO

Name: Cold sensitive U2 snRNA suppressor 1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [11]

Biological process:
U2-type prespliceosome assembly spliceosomal complex assembly mRNA splicing, via spliceosome
Molecular function:
RNA binding
Cellular location:
spliceosomal complex nucleus U2 snRNP U2-type prespliceosome catalytic step 2 spliceosome precatalytic spliceosome U2-type spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	50 kDa	-

LEVO

Name: Pre-mRNA-splicing factor PRP11
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [10]

Biological process:
mRNA splicing, via spliceosome U2-type prespliceosome assembly spliceosomal complex assembly
Molecular function:
zinc ion binding RNA binding
Cellular location:
nucleus spliceosomal complex U2-type prespliceosome U2 snRNP catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	29 kDa	-

LEVO

Name: Pre-mRNA-splicing factor RDS3
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [9]

Biological process:
U2-type prespliceosome assembly mRNA splicing, via spliceosome spliceosomal complex assembly response to xenobiotic stimulus

Cellular location:
nucleus spliceosomal complex precatalytic spliceosome U2 snRNP U2-type spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	12 kDa	-

LEVO

Name: RDS3 complex subunit 10
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [8]

Biological process:
U2-type prespliceosome assembly mRNA splicing, via spliceosome spliceosomal complex assembly

Cellular location:
nucleus spliceosomal complex U2 snRNP precatalytic spliceosome U2-type spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	10 kDa	-

LEVO

Name: Pre-mRNA-splicing factor PRP46
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [5]

Biological process:
mRNA splicing, via spliceosome

Cellular location:
Prp19 complex catalytic step 2 spliceosome spliceosomal complex cytoplasm

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	50 kDa	-

LEVO

Name: Pre-mRNA-processing protein 45
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [5]

Biological process:
generation of catalytic spliceosome for second transesterification step

Cellular location:
U2-type catalytic step 2 spliceosome U2-type catalytic step 1 spliceosome nucleus post-mRNA release spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	42 kDa	-

LEVO

Name: Pre-mRNA-splicing factor SLT11
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [7]

Biological process:
mRNA splicing, via spliceosome
Molecular function:
pre-mRNA binding U6 snRNA binding
Cellular location:
nucleus U2-type catalytic step 2 spliceosome U2-type catalytic step 1 spliceosome Prp19 complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	40 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC2
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [12]

Biological process:
positive regulation of RNA splicing mRNA splicing, via spliceosome spliceosomal snRNP assembly positive regulation of cell cycle mRNA cis splicing, via spliceosome cell cycle
Molecular function:
metal ion binding U6 snRNA binding pre-mRNA binding
Cellular location:
Prp19 complex U2-type catalytic step 1 spliceosome U2-type catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	38 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC15
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [6]

Biological process:
mRNA splicing, via spliceosome mRNA cis splicing, via spliceosome
Molecular function:
RNA binding
Cellular location:
catalytic step 2 spliceosome U2-type spliceosomal complex nucleus

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	19 kDa	-

LEVO

Name: Pre-mRNA-splicing factor BUD31
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [5]

Biological process:
cellular bud site selection mRNA splicing, via spliceosome

Cellular location:
nucleus U2 snRNP spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	18 kDa	-

LEVO

Name: Pre-mRNA leakage protein 1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [10]

Biological process:
U2-type prespliceosome assembly maintenance of RNA location mRNA splicing, via spliceosome mRNA export from nucleus
Molecular function:
mRNA binding
Cellular location:
spliceosomal complex U2 snRNP nucleus cytoplasm RES complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	24 kDa	-

LEVO

Name: U2 snRNP component IST3
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [13]

Biological process:
U2-type prespliceosome assembly maintenance of RNA location spliceosomal complex assembly mRNA export from nucleus mRNA splicing, via spliceosome generation of catalytic spliceosome for first transesterification step
Molecular function:
RNA binding
Cellular location:
spliceosomal complex nucleus precatalytic spliceosome cytoplasm U2 snRNP RES complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	17 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC26
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [9]

Biological process:
U2-type prespliceosome assembly maintenance of RNA location mRNA splicing, via spliceosome

Cellular location:
spliceosomal complex U2 snRNP cytoplasm RES complex U2-type spliceosomal complex nucleus

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	30 kDa	-

LEVO

Name: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
EC number: 3.6.4.13
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [9]

Biological process:
generation of catalytic spliceosome for first transesterification step snoRNA splicing
Molecular function:
helicase activity ATP hydrolysis activity RNA helicase activity ATP binding RNA binding ATP-dependent activity, acting on RNA
Cellular location:
U2-type catalytic step 1 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	99 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC22
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [5]

Biological process:
mRNA splicing, via spliceosome
Molecular function:
RNA binding
Cellular location:
U2-type spliceosomal complex catalytic step 2 spliceosome cytoplasm

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	67 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC24
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [8]

Biological process:
snoRNA splicing generation of catalytic spliceosome for first transesterification step mRNA splicing, via spliceosome
Molecular function:
DNA binding metal ion binding
Cellular location:
nucleus spliceosomal complex U2-type spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	29 kDa	-

LEVO

Name: Peptidyl-prolyl isomerase CWC27
EC number: 5.2.1.8
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [7]

Biological process:
protein peptidyl-prolyl isomerization RNA splicing mRNA processing
Molecular function:
peptidyl-prolyl cis-trans isomerase activity
Cellular location:
catalytic step 2 spliceosome U2-type spliceosomal complex cytoplasm

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	35 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CEF1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	61 kDa	-

LEVO

Name: U2 snRNP component HSH155
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [10]

Biological process:
U2-type prespliceosome assembly spliceosomal complex assembly mRNA splicing, via spliceosome
Molecular function:
mRNA binding
Cellular location:
nucleus spliceosomal complex U2-type spliceosomal complex U2 snRNP U2-type prespliceosome catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	110 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CLF1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB

Natural source

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	68 kDa	-

LEVO

Name: Pre-mRNA-splicing factor CWC21
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [3]

Biological process:
mRNA splicing, via spliceosome

Cellular location:
cytoplasm U2-type spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	15 kDa	-

LEVO

Name: Pre-mRNA-splicing factor SYF1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Natural source

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	78 kDa	-

LEVO

Name: Protein HSH49
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [13]

Biological process:
U2-type prespliceosome assembly mRNA splicing, via spliceosome
Molecular function:
mRNA 3'-UTR binding poly(A) binding RNA binding poly(U) RNA binding
Cellular location:
spliceosomal complex U2-type prespliceosome ribonucleoprotein complex U2 snRNP nucleus U2-type spliceosomal complex cytosol

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	24 kDa	-

LEVO

Name: Pre-mRNA-splicing factor SYF2
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [8]

Biological process:
mRNA splicing, via spliceosome

Cellular location:
cytosol nucleus U2-type post-mRNA release spliceosomal complex U2-type catalytic step 1 spliceosome Prp19 complex U2-type catalytic step 2 spliceosome U2-type prespliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	24 kDa	-

LEVO

Name: Pre-mRNA-processing factor 19
EC number: 6.3.2.-
Number of copies: 4
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [13]

Biological process:
protein K63-linked ubiquitination generation of catalytic spliceosome for first transesterification step DNA repair mRNA splicing, via spliceosome
Molecular function:
identical protein binding ubiquitin-protein transferase activity ubiquitin protein ligase activity
Cellular location:
nucleus U2-type catalytic step 1 spliceosome mitochondrion Prp19 complex cytoplasm cytosol

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	56 kDa	-

LEVO

Name: Pre-mRNA-splicing factor SNT309
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Gene Ontology [3]

Biological process:
mRNA splicing, via spliceosome

Cellular location:
Prp19 complex U2-type catalytic step 1 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	20 kDa	-

LEVO

Name: Pre-mRNA-processing factor 17
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [8]

Biological process:
mRNA branch site recognition mRNA splicing, via spliceosome generation of catalytic spliceosome for second transesterification step mRNA 3'-splice site recognition
Molecular function:
mRNA binding
Cellular location:
nuclear periphery catalytic step 2 spliceosome spliceosomal complex

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	52 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein-associated protein B
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [19]

Biological process:
mRNA 5'-splice site recognition U2-type prespliceosome assembly spliceosomal complex assembly spliceosomal snRNP assembly 7-methylguanosine cap hypermethylation mRNA splicing, via spliceosome
Molecular function:
RNA binding
Cellular location:
U4/U6 snRNP spliceosomal complex commitment complex nucleus U4 snRNP catalytic step 2 spliceosome U5 snRNP U4/U6 x U5 tri-snRNP complex U2-type prespliceosome U1 snRNP cytoplasm U2 snRNP

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	22 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein E
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [21]

Biological process:
mRNA 5'-splice site recognition U2-type prespliceosome assembly spliceosomal complex assembly spliceosomal snRNP assembly mRNA splicing, via spliceosome 7-methylguanosine cap hypermethylation
Molecular function:
splicing factor binding RNA binding
Cellular location:
pICln-Sm protein complex commitment complex nucleus spliceosomal complex U4/U6 snRNP cytoplasm U4/U6 x U5 tri-snRNP complex U2-type prespliceosome U4 snRNP U1 snRNP U2 snRNP precatalytic spliceosome U5 snRNP

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	10 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein F
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [21]

Biological process:
spliceosomal complex assembly U2-type prespliceosome assembly mRNA 5'-splice site recognition spliceosomal snRNP assembly 7-methylguanosine cap hypermethylation mRNA splicing, via spliceosome
Molecular function:
poly(U) RNA binding RNA binding splicing factor binding
Cellular location:
pICln-Sm protein complex U4/U6 snRNP U4 snRNP nucleus commitment complex spliceosomal complex U4/U6 x U5 tri-snRNP complex cytoplasm U2 snRNP U5 snRNP U1 snRNP catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	9 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein G
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [24]

Biological process:
mRNA 5'-splice site recognition U2-type prespliceosome assembly spliceosomal complex assembly 7-methylguanosine cap hypermethylation spliceosomal snRNP assembly mRNA splicing, via spliceosome
Molecular function:
RNA binding
Cellular location:
nucleus commitment complex cytoplasm U4/U6 snRNP spliceosomal complex SMN-Sm protein complex U4/U6 x U5 tri-snRNP complex U4 snRNP U1 snRNP U2-type prespliceosome U5 snRNP U2 snRNP U12-type spliceosomal complex ribonucleoprotein complex spliceosomal tri-snRNP complex catalytic step 2 spliceosome precatalytic spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	8 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein Sm D3
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [26]

Biological process:
spliceosomal complex assembly mRNA 5'-splice site recognition U2-type prespliceosome assembly spliceosomal snRNP assembly 7-methylguanosine cap hypermethylation mRNA splicing, via spliceosome
Molecular function:
mRNA binding RNA binding
Cellular location:
U4/U6 snRNP cytoplasm spliceosomal complex nucleus spliceosomal tri-snRNP complex precatalytic spliceosome pICln-Sm protein complex U12-type spliceosomal complex U4 snRNP U2-type prespliceosome U2 snRNP SMN-Sm protein complex U1 snRNP U5 snRNP U4/U6 x U5 tri-snRNP complex catalytic step 2 spliceosome commitment complex cytosol

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	11 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein Sm D1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [27]

Biological process:
spliceosomal complex assembly mRNA 5'-splice site recognition U2-type prespliceosome assembly 7-methylguanosine cap hypermethylation spliceosomal snRNP assembly mRNA splicing, via spliceosome
Molecular function:
RNA binding mRNA binding
Cellular location:
spliceosomal complex cytoplasm U4/U6 snRNP spliceosomal tri-snRNP complex commitment complex nucleus U5 snRNP U1 snRNP U4 snRNP U2-type prespliceosome U2 snRNP cytosol precatalytic spliceosome pICln-Sm protein complex U4/U6 x U5 tri-snRNP complex small nuclear ribonucleoprotein complex SMN-Sm protein complex U12-type spliceosomal complex catalytic step 2 spliceosome

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	16 kDa	-

LEVO

Name: Small nuclear ribonucleoprotein Sm D2
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB

Domains

Gene Ontology [1]

Molecular function:
RNA binding

Natural source

Organism: Baker's yeast
Strain: ATCC 204508 / S288c

Molecular weight

Experimental	Theoretical	Method
-	11 kDa	-

RNA [5]

Name: Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence
Number of copies: 1

Natural source

Organism: Saccharomyces cerevisiae

Molecular weight

Experimental	Theoretical	Method
-	68 kDa	-

Name: Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence
Number of copies: 1

Natural source

Organism: Saccharomyces cerevisiae

Molecular weight

Experimental	Theoretical	Method
-	35 kDa	-

Name: U2 snRNA
Number of copies: 1

Natural source

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	376 kDa	-

Name: Pre-mRNA
Number of copies: 1

Natural source

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	7 kDa	-

Name: Pre-mRNA
Number of copies: 1

Natural source

Organism: Saccharomyces cerevisiae S288c

Molecular weight

Experimental	Theoretical	Method
-	18 kDa	-

Ligand [4]

Name: GUANOSINE-5'-TRIPHOSPHATE
HET code: GTP
Number of copies: 1
ChEMBL ChEBI DrugBank

Molecular weight

Experimental	Theoretical	Method
-	523 Da	-

Name: MAGNESIUM ION
HET code: MG
Number of copies: 5
ChEBI DrugBank

Molecular weight

Experimental	Theoretical	Method
-	24 Da	-

Name: ZINC ION
HET code: ZN
Number of copies: 13
ChEMBL ChEBI DrugBank

Molecular weight

Experimental	Theoretical	Method
-	65 Da	-

Name: ADENOSINE-5'-DIPHOSPHATE
HET code: ADP
Number of copies: 1
ChEMBL ChEBI DrugBank

Molecular weight

Experimental	Theoretical	Method
-	427 Da	-