EMDB EMD-9524

Single particle reconstruction
3.5Å resolution

Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution

Map released:
Last modified:
Overview of EMD-9524
Source organisms:
Fitted atomic model: 5gm6
Primary publication:
Structure of a yeast activated spliceosome at 3.5 angstrom resolution
Yan C, Wan R, Bai R, Huang G, Shi Y
Science 353 904-911 (2016)
PMID: 27445306

Function and Biology Details

Sample name: Bact spliceosomal complex
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ZINC ION, ADENOSINE-5'-DIPHOSPHATE
Nucleic acids: Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence, Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence, U2 snRNA, Pre-mRNA, Pre-mRNA
Proteins: Bact spliceosomal complex, Pre-mRNA-splicing factor 8, Pre-mRNA-splicing helicase BRR2, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor RSE1, Cold sensitive U2 snRNA suppressor 1, Pre-mRNA-splicing factor PRP11, Pre-mRNA-splicing factor RDS3, RDS3 complex subunit 10, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor BUD31, Pre-mRNA leakage protein 1, U2 snRNP component IST3, Pre-mRNA-splicing factor CWC26, Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2, Pre-mRNA-splicing factor CWC22, Pre-mRNA-splicing factor CWC24, Peptidyl-prolyl isomerase CWC27, Pre-mRNA-splicing factor CEF1, U2 snRNP component HSH155, Pre-mRNA-splicing factor CLF1, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor SYF1, Protein HSH49, Pre-mRNA-splicing factor SYF2, Pre-mRNA-processing factor 19, Pre-mRNA-splicing factor SNT309, Pre-mRNA-processing factor 17, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)