Single particle reconstruction
6.35Å resolution

Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry

Map released:
Overview of EMD-9316
Source organisms:
Fitted atomic model: 6n1p
Related EM entry by publication: EMD-9317, EMD-9318
Primary publication:
CryoEM structures of open dimers of Gyrase A in complex with DNA illuminate mechanism of strand passage.
Soczek KM, Grant T, Rosenthal PB, Mondragon A
Elife 7 - (2018)
PMID: 30457554

Function and Biology Details

Sample name: Complex of DNA Gyrase A subunit with DNA in C2 symmetry
Nucleic acids: DNA (44-MER), DNA (44-MER)
Proteins: Complex of DNA Gyrase A subunit with DNA in C2 symmetry, DNA Gyrase A, DNA, DNA gyrase subunit A

Experimental Information Details

Resolution: 6.35Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: JEOL 3200FS
Detector: GATAN K2 SUMMIT (4k x 4k)