Sample name: Bacterial microcompartment shell from Haliangium ochraceum
Cellular component: Bacterial microcompartment shell from Haliangium ochraceum
Proteins: Microcompartments protein, Microcompartments protein
Applied symmetry: C1
Number of particles: 177642
Reconstruction protocol: FOURIER SPACE
CTF correction: Initial CTF fitting using CTFFIND4, CTF correction applied within RELION
Details: The dataset was refined without masking and subsequently masked to reveal only the subregion of the BMC shell to which the focused classification had been applied.
Initial atomic models: 5V74, 5DIH
Refinement space: REAL
Microscope model: FEI TITAN
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 25.0
Nominal CS (mm): 2.70
Holder model: GATAN LIQUID NITROGEN
Number of images: 928
Sampling interval (μm): 5.0
Details: 928 images were retained after inspection for image quality.
Specimen state: particle
Specimen concentration (mg/mL): 3.00
Specimen support: Protochips C-flat 1.2/1.3 holey carbon grids were coated with a thin carbon film and plasma-cleaned using a Gatan Solarus.
Apparatus: FEI VITROBOT MARK IV
Details: 5-7 second incubation of the sample on the grid before blotting and plunging.