EMD-9245

Single-particle
4.2 Å
EMD-9245
3D View Gallery
Deposition: 22/10/2018
Map released: 05/12/2018
Last modified: 25/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9245
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of full-length IP3R1 channel in ligand-bound state

EMD-9245

Single-particle
4.2 Å
EMD-9245
3D View Gallery
Deposition: 22/10/2018
Map released: 05/12/2018
Last modified: 25/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Details: 0.1 mg/ml
Buffer
pH: 7.4
Details: 50 mM Tris-HCl buffer (pH 7.4), 150 mM NaCl, 1 mM DTT, 0.4% CHAPS,100 nM of AdA, 300 nM of Ca2+, protease inhibitors
Grid
Details: Unavailable
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 293 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.0 mm
Specimen holder model: GATAN 910 MULTI-SPECIMEN SINGLE TILT CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Temperature
Minimum: 93.0 K
Maximum: 93.0 K
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Frames per image: 2-17
Number of real images: 14686
Average exposure time: 0.2 s
Average electron dose per image: 1.3 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 38405
Algorithm: FOURIER SPACE
Software [1]
Name Version Details
EMAN2 2.1 -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-6369
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
EMAN2 2.1 -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
EMAN2 2.1 -
Particle selection [1]
Selected Ref. model Method Software Details
191646 - - - -
Final 3D classification
Software [1]
Name Version Details
EMAN2 2.1 -
CTF correction
Software [1]
Name Version Details
EMAN2 2.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM density map of IP3R1 in ligand-bound state
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.26 Å 1.26 Å 1.26 Å
Contour list
Primary Level Source
True 0.045 AUTHOR