EMD-9243

Single-particle
4.1 Å
EMD-9243
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Deposition: 22/10/2018
Map released: 05/12/2018
Last modified: 27/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9243
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6mu1 summary report (pdf.gz) Map and 6mu1 FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of full-length IP3R1 channel bound with Adenophostin A (composite)

EMD-9243

Single-particle
4.1 Å
EMD-9243
3D View Gallery
Deposition: 22/10/2018
Map released: 05/12/2018
Last modified: 27/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.4
Details: 50 mM Tris-HCl buffer (pH 7.4), 150 mM NaCl, 1 mM DTT, 0.4% CHAPS,100 nM of AdA, 300 nM of Ca2+, protease inhibitors
Grid
Mesh: 400
Model: Quantifoil R2/1
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 293 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.0 mm
Specimen holder model: GATAN 910 MULTI-SPECIMEN SINGLE TILT CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Temperature
Minimum: 93.0 K
Maximum: 93.0 K
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Frames per image: 2-17
Number of real images: 14686
Average exposure time: 0.2 s
Average electron dose per image: 1.3 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 179760
⌯ Applied Symmetry
Point group: C4
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-6369
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 1.4 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 1.4 -
Particle selection [1]
Selected Ref. model Method Software Details
191646 - - - -
Final 3D classification
Number of classes: 6
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Software [1]
Name Version Details
CTFFIND 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM density map (composite) of IP3R1 in ligand-bound state
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.26 Å 1.26 Å 1.26 Å
Contour list
Primary Level Source
True 1.75 AUTHOR