EMD-9216
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
EMD-9216
Single-particle3.0 Å
Deposition: 16/10/2018
Map released: 05/12/2018
Last modified: 18/12/2019
Name: Human 26S proteasome
Summary:
- Complex
- Protein
- 26S proteasome non-ATPase regulatory subunit 1
- 26S proteasome non-ATPase regulatory subunit 3
- 26S proteasome non-ATPase regulatory subunit 12
- 26S proteasome non-ATPase regulatory subunit 11
- 26S proteasome non-ATPase regulatory subunit 6
- 26S proteasome non-ATPase regulatory subunit 7
- 26S proteasome non-ATPase regulatory subunit 13
- 26S proteasome non-ATPase regulatory subunit 4
- 26S proteasome non-ATPase regulatory subunit 14
- 26S proteasome non-ATPase regulatory subunit 8
- 26S proteasome complex subunit SEM1
- 26S proteasome non-ATPase regulatory subunit 2
- 26S proteasome regulatory subunit 7
- 26S proteasome regulatory subunit 4
- 26S proteasome regulatory subunit 8
- 26S proteasome regulatory subunit 6B
- 26S proteasome regulatory subunit 10B
- 26S proteasome regulatory subunit 6A
- Ubiquitin
- Proteasome subunit alpha type-6
- Proteasome subunit alpha type-2
- Proteasome subunit alpha type-4
- Proteasome subunit alpha type-7
- Proteasome subunit alpha type-5
- Proteasome subunit alpha type-1
- Proteasome subunit alpha type-3
- Proteasome subunit beta type-6
- Proteasome subunit beta type-7
- Proteasome subunit beta type-3
- Proteasome subunit beta type-2
- Proteasome subunit beta type-5
- Proteasome subunit beta type-1
- Proteasome subunit beta type-4
- Ligand
Human 26S proteasome
Name: Natural source [1]
Organism: Homo sapiens
Recombinant Expression [1]
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Complex Portal [2]
ID | Name | Overlap |
---|---|---|
CPX-5993 | 26S Proteasome complex | 0.74 |
CPX-5993 | 26S Proteasome complex | 0.76 |
26S proteasome non-ATPase regulatory subunit 1
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 105 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 3
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 61 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 12
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
membrane proteasome regulatory particle, lid subcomplex proteasome complex extracellular exosome nuclear proteasome complex cytoplasm proteasome accessory complex secretory granule lumen ficolin-1-rich granule lumen extracellular region proteasome regulatory particle cytosol nucleoplasm
proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
membrane proteasome regulatory particle, lid subcomplex proteasome complex extracellular exosome nuclear proteasome complex cytoplasm proteasome accessory complex secretory granule lumen ficolin-1-rich granule lumen extracellular region proteasome regulatory particle cytosol nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 52 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 11
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process stem cell differentiation proteasome assembly
Molecular function:
structural molecule activity
Cellular location:
secretory granule lumen proteasome accessory complex membrane ficolin-1-rich granule lumen proteasome regulatory particle, lid subcomplex nucleus nucleoplasm extracellular region proteasome complex cytosol
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process stem cell differentiation proteasome assembly
Molecular function:
structural molecule activity
Cellular location:
secretory granule lumen proteasome accessory complex membrane ficolin-1-rich granule lumen proteasome regulatory particle, lid subcomplex nucleus nucleoplasm extracellular region proteasome complex cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 47 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 6
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [10]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
enzyme regulator activity
Cellular location:
proteasome accessory complex nucleoplasm proteasome complex cytosol ficolin-1-rich granule lumen secretory granule lumen extracellular region proteasome regulatory particle
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
enzyme regulator activity
Cellular location:
proteasome accessory complex nucleoplasm proteasome complex cytosol ficolin-1-rich granule lumen secretory granule lumen extracellular region proteasome regulatory particle
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 45 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 7
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
protein homodimerization activity
Cellular location:
proteasome regulatory particle proteasome complex membrane extracellular region extracellular exosome secretory granule lumen nucleoplasm cytosol ficolin-1-rich granule lumen nucleus
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
protein homodimerization activity
Cellular location:
proteasome regulatory particle proteasome complex membrane extracellular region extracellular exosome secretory granule lumen nucleoplasm cytosol ficolin-1-rich granule lumen nucleus
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 37 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 13
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process meiosis I ubiquitin-dependent protein catabolic process
Molecular function:
structural molecule activity
Cellular location:
cytosol secretory granule lumen ficolin-1-rich granule lumen nucleoplasm extracellular region nucleus proteasome complex proteasome regulatory particle membrane proteasome regulatory particle, lid subcomplex proteasome accessory complex
proteasome-mediated ubiquitin-dependent protein catabolic process meiosis I ubiquitin-dependent protein catabolic process
Molecular function:
structural molecule activity
Cellular location:
cytosol secretory granule lumen ficolin-1-rich granule lumen nucleoplasm extracellular region nucleus proteasome complex proteasome regulatory particle membrane proteasome regulatory particle, lid subcomplex proteasome accessory complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 42 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 4
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [10]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
RNA binding identical protein binding polyubiquitin modification-dependent protein binding
Cellular location:
proteasome regulatory particle, base subcomplex cytosol nucleoplasm nucleus proteasome accessory complex proteasome complex
proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
RNA binding identical protein binding polyubiquitin modification-dependent protein binding
Cellular location:
proteasome regulatory particle, base subcomplex cytosol nucleoplasm nucleus proteasome accessory complex proteasome complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 40 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 14
EC number: 3.4.19.-
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.19.-
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [26]
Biological process:
regulation of proteasomal protein catabolic process response to ethanol protein K63-linked deubiquitination double-strand break repair via homologous recombination proteasome-mediated ubiquitin-dependent protein catabolic process double-strand break repair via nonhomologous end joining protein deubiquitination ubiquitin-dependent protein catabolic process
Molecular function:
K63-linked deubiquitinase activity isopeptidase activity metallopeptidase activity Lys63-specific deubiquitinase activity endopeptidase activator activity metal-dependent deubiquitinase activity proteasome binding metal ion binding
Cellular location:
cytosolic proteasome complex proteasome complex nucleoplasm secretory granule lumen proteasome accessory complex proteasome regulatory particle, lid subcomplex extracellular region ficolin-1-rich granule lumen nucleus cytosol
regulation of proteasomal protein catabolic process response to ethanol protein K63-linked deubiquitination double-strand break repair via homologous recombination proteasome-mediated ubiquitin-dependent protein catabolic process double-strand break repair via nonhomologous end joining protein deubiquitination ubiquitin-dependent protein catabolic process
Molecular function:
K63-linked deubiquitinase activity isopeptidase activity metallopeptidase activity Lys63-specific deubiquitinase activity endopeptidase activator activity metal-dependent deubiquitinase activity proteasome binding metal ion binding
Cellular location:
cytosolic proteasome complex proteasome complex nucleoplasm secretory granule lumen proteasome accessory complex proteasome regulatory particle, lid subcomplex extracellular region ficolin-1-rich granule lumen nucleus cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 34 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 8
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [8]
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 39 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome complex subunit SEM1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [9]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process mRNA export from nucleus double-strand break repair via homologous recombination proteasome assembly
Cellular location:
proteasome complex protein-containing complex proteasome regulatory particle, lid subcomplex nucleoplasm cytosol
proteasome-mediated ubiquitin-dependent protein catabolic process mRNA export from nucleus double-strand break repair via homologous recombination proteasome assembly
Cellular location:
proteasome complex protein-containing complex proteasome regulatory particle, lid subcomplex nucleoplasm cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 8 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome non-ATPase regulatory subunit 2
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [16]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein catabolic process
Molecular function:
enzyme regulator activity
Cellular location:
cytosol proteasome accessory complex nucleus proteasome regulatory particle, base subcomplex proteasome regulatory particle proteasome storage granule extracellular region nucleoplasm extracellular exosome membrane secretory granule lumen proteasome complex ficolin-1-rich granule lumen
proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein catabolic process
Molecular function:
enzyme regulator activity
Cellular location:
cytosol proteasome accessory complex nucleus proteasome regulatory particle, base subcomplex proteasome regulatory particle proteasome storage granule extracellular region nucleoplasm extracellular exosome membrane secretory granule lumen proteasome complex ficolin-1-rich granule lumen
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 100 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 7
Number of copies: 1
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [20]
Biological process:
osteoblast differentiation positive regulation of proteasomal protein catabolic process ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
ATP hydrolysis activity ATP binding proteasome-activating activity
Cellular location:
membrane nucleus proteasome complex ficolin-1-rich granule lumen secretory granule lumen P-body cytoplasm cytoplasmic ribonucleoprotein granule nucleoplasm proteasome accessory complex proteasome regulatory particle, base subcomplex extracellular region cytosol
osteoblast differentiation positive regulation of proteasomal protein catabolic process ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
ATP hydrolysis activity ATP binding proteasome-activating activity
Cellular location:
membrane nucleus proteasome complex ficolin-1-rich granule lumen secretory granule lumen P-body cytoplasm cytoplasmic ribonucleoprotein granule nucleoplasm proteasome accessory complex proteasome regulatory particle, base subcomplex extracellular region cytosol
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 48 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 4
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 49 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 8
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [31]
Biological process:
positive regulation of inclusion body assembly modulation of chemical synaptic transmission negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of programmed cell death positive regulation of proteasomal protein catabolic process regulation of transcription by RNA polymerase II
Molecular function:
ATP hydrolysis activity DNA-binding transcription factor binding ATP binding thyrotropin-releasing hormone receptor binding TBP-class protein binding transcription factor binding general transcription initiation factor binding proteasome-activating activity
Cellular location:
proteasome accessory complex cytoplasm proteasome complex postsynapse cytosolic proteasome complex cytoplasmic vesicle nucleus cytosol membrane extracellular exosome inclusion body proteasome regulatory particle, base subcomplex nuclear proteasome complex blood microparticle nucleoplasm
positive regulation of inclusion body assembly modulation of chemical synaptic transmission negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of programmed cell death positive regulation of proteasomal protein catabolic process regulation of transcription by RNA polymerase II
Molecular function:
ATP hydrolysis activity DNA-binding transcription factor binding ATP binding thyrotropin-releasing hormone receptor binding TBP-class protein binding transcription factor binding general transcription initiation factor binding proteasome-activating activity
Cellular location:
proteasome accessory complex cytoplasm proteasome complex postsynapse cytosolic proteasome complex cytoplasmic vesicle nucleus cytosol membrane extracellular exosome inclusion body proteasome regulatory particle, base subcomplex nuclear proteasome complex blood microparticle nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 44 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 6B
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
blastocyst development proteolysis proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
ATP hydrolysis activity ATP binding proteasome-activating activity
Cellular location:
nucleus proteasome regulatory particle, base subcomplex proteasome complex cytosol nucleoplasm proteasome accessory complex membrane
blastocyst development proteolysis proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
ATP hydrolysis activity ATP binding proteasome-activating activity
Cellular location:
nucleus proteasome regulatory particle, base subcomplex proteasome complex cytosol nucleoplasm proteasome accessory complex membrane
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 47 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 10B
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [20]
Biological process:
positive regulation of inclusion body assembly positive regulation of proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent ERAD pathway positive regulation of RNA polymerase II transcription preinitiation complex assembly
Molecular function:
ATP binding identical protein binding proteasome-activating activity protein-macromolecule adaptor activity ATP hydrolysis activity
Cellular location:
extracellular exosome membrane cytosolic proteasome complex proteasome regulatory particle, base subcomplex inclusion body proteasome complex cytosol nucleus nucleoplasm proteasome accessory complex
positive regulation of inclusion body assembly positive regulation of proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent ERAD pathway positive regulation of RNA polymerase II transcription preinitiation complex assembly
Molecular function:
ATP binding identical protein binding proteasome-activating activity protein-macromolecule adaptor activity ATP hydrolysis activity
Cellular location:
extracellular exosome membrane cytosolic proteasome complex proteasome regulatory particle, base subcomplex inclusion body proteasome complex cytosol nucleus nucleoplasm proteasome accessory complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 45 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
26S proteasome regulatory subunit 6A
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [19]
Biological process:
positive regulation of proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of transcription by RNA polymerase II modulation by host of viral transcription
Molecular function:
ATP binding proteasome-activating activity ATP hydrolysis activity identical protein binding
Cellular location:
ficolin-1-rich granule lumen P-body membrane secretory granule lumen nucleus proteasome regulatory particle, base subcomplex proteasome accessory complex proteasome complex nucleoplasm cytosol extracellular region
positive regulation of proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of transcription by RNA polymerase II modulation by host of viral transcription
Molecular function:
ATP binding proteasome-activating activity ATP hydrolysis activity identical protein binding
Cellular location:
ficolin-1-rich granule lumen P-body membrane secretory granule lumen nucleus proteasome regulatory particle, base subcomplex proteasome accessory complex proteasome complex nucleoplasm cytosol extracellular region
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 49 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Ubiquitin
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 8 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-6
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [25]
Biological process:
proteolysis involved in protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process proteolysis involved in cellular protein catabolic process regulation of inflammatory response ubiquitin-dependent protein catabolic process positive regulation of NF-kappaB transcription factor activity
Molecular function:
purine ribonucleoside triphosphate binding endopeptidase activity NF-kappaB binding RNA binding
Cellular location:
ribosome cytoplasm nuclear matrix proteasome core complex, alpha-subunit complex myofibril polysome proteasome core complex nucleoplasm cytosol proteasome complex extracellular exosome P-body nucleus sarcomere
proteolysis involved in protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process proteolysis involved in cellular protein catabolic process regulation of inflammatory response ubiquitin-dependent protein catabolic process positive regulation of NF-kappaB transcription factor activity
Molecular function:
purine ribonucleoside triphosphate binding endopeptidase activity NF-kappaB binding RNA binding
Cellular location:
ribosome cytoplasm nuclear matrix proteasome core complex, alpha-subunit complex myofibril polysome proteasome core complex nucleoplasm cytosol proteasome complex extracellular exosome P-body nucleus sarcomere
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 27 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-2
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasomal protein catabolic process response to virus proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
cytoplasm P-body proteasome core complex, alpha-subunit complex extracellular exosome proteasome complex cytosol proteasome core complex secretory granule lumen nucleus extracellular region nucleoplasm ficolin-1-rich granule lumen
proteasomal protein catabolic process response to virus proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
cytoplasm P-body proteasome core complex, alpha-subunit complex extracellular exosome proteasome complex cytosol proteasome core complex secretory granule lumen nucleus extracellular region nucleoplasm ficolin-1-rich granule lumen
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 25 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-4
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
threonine-type endopeptidase activity
Cellular location:
cytoplasm proteasome complex nucleus proteasome core complex cytosol nucleoplasm proteasome core complex, alpha-subunit complex intracellular membrane-bounded organelle P-body extracellular exosome
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
threonine-type endopeptidase activity
Cellular location:
cytoplasm proteasome complex nucleus proteasome core complex cytosol nucleoplasm proteasome core complex, alpha-subunit complex intracellular membrane-bounded organelle P-body extracellular exosome
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 29 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-7
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
identical protein binding
Cellular location:
cytoplasm extracellular exosome proteasome complex proteasome core complex, alpha-subunit complex nucleus postsynapse cytosol nucleoplasm proteasome core complex
proteasome-mediated ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
identical protein binding
Cellular location:
cytoplasm extracellular exosome proteasome complex proteasome core complex, alpha-subunit complex nucleus postsynapse cytosol nucleoplasm proteasome core complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 27 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-5
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Cellular location:
cytoplasm nucleus proteasome core complex proteasome core complex, alpha-subunit complex secretory granule lumen extracellular region cytosol ficolin-1-rich granule lumen proteasome complex extracellular exosome nucleoplasm
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Cellular location:
cytoplasm nucleus proteasome core complex proteasome core complex, alpha-subunit complex secretory granule lumen extracellular region cytosol ficolin-1-rich granule lumen proteasome complex extracellular exosome nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 26 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-1
EC number: 3.4.25.1
Number of copies: 2
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process immune system process negative regulation of inflammatory response to antigenic stimulus
Molecular function:
lipopolysaccharide binding RNA binding
Cellular location:
centrosome nucleoplasm polysome proteasome core complex, alpha-subunit complex proteasome core complex extracellular exosome nucleus cytosol proteasome complex
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process immune system process negative regulation of inflammatory response to antigenic stimulus
Molecular function:
lipopolysaccharide binding RNA binding
Cellular location:
centrosome nucleoplasm polysome proteasome core complex, alpha-subunit complex proteasome core complex extracellular exosome nucleus cytosol proteasome complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 30 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit alpha type-3
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process regulation of endopeptidase activity proteasomal protein catabolic process ubiquitin-dependent protein catabolic process
Molecular function:
ubiquitin protein ligase binding
Cellular location:
nucleus proteasome core complex, alpha-subunit complex cytoplasm synapse cytosol extracellular exosome proteasome complex nucleoplasm proteasome core complex
proteasome-mediated ubiquitin-dependent protein catabolic process regulation of endopeptidase activity proteasomal protein catabolic process ubiquitin-dependent protein catabolic process
Molecular function:
ubiquitin protein ligase binding
Cellular location:
nucleus proteasome core complex, alpha-subunit complex cytoplasm synapse cytosol extracellular exosome proteasome complex nucleoplasm proteasome core complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 28 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-6
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
threonine-type endopeptidase activity cadherin binding endopeptidase activity
Cellular location:
mitochondrion cytoplasm cytosol proteasome complex extracellular exosome proteasome core complex, beta-subunit complex nucleus proteasome core complex nucleoplasm
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
threonine-type endopeptidase activity cadherin binding endopeptidase activity
Cellular location:
mitochondrion cytoplasm cytosol proteasome complex extracellular exosome proteasome core complex, beta-subunit complex nucleus proteasome core complex nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 25 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-7
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
endopeptidase activity threonine-type endopeptidase activity
Cellular location:
cytoplasm secretory granule lumen proteasome core complex nuclear body proteasome core complex, beta-subunit complex cytosol proteasome complex nucleus extracellular region nucleoplasm ficolin-1-rich granule lumen
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process
Molecular function:
endopeptidase activity threonine-type endopeptidase activity
Cellular location:
cytoplasm secretory granule lumen proteasome core complex nuclear body proteasome core complex, beta-subunit complex cytosol proteasome complex nucleus extracellular region nucleoplasm ficolin-1-rich granule lumen
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 29 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-3
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 22 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-2
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process response to organonitrogen compound response to organic cyclic compound
Molecular function:
threonine-type endopeptidase activity peptidase activity
Cellular location:
cytoplasm proteasome core complex, beta-subunit complex extracellular exosome proteasome complex nucleus proteasome core complex membrane cytosol nucleoplasm
proteasome-mediated ubiquitin-dependent protein catabolic process proteasomal protein catabolic process response to organonitrogen compound response to organic cyclic compound
Molecular function:
threonine-type endopeptidase activity peptidase activity
Cellular location:
cytoplasm proteasome core complex, beta-subunit complex extracellular exosome proteasome complex nucleus proteasome core complex membrane cytosol nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 22 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-5
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [16]
Biological process:
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process response to oxidative stress proteolysis
Molecular function:
peptidase activity endopeptidase activity threonine-type endopeptidase activity
Cellular location:
cytoplasm extracellular exosome nucleus centrosome cytosol proteasome core complex, beta-subunit complex nucleoplasm proteasome complex proteasome core complex
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process response to oxidative stress proteolysis
Molecular function:
peptidase activity endopeptidase activity threonine-type endopeptidase activity
Cellular location:
cytoplasm extracellular exosome nucleus centrosome cytosol proteasome core complex, beta-subunit complex nucleoplasm proteasome complex proteasome core complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 28 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-1
EC number: 3.4.25.1
Number of copies: 2
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
cytoplasm extracellular exosome proteasome core complex, beta-subunit complex secretory granule lumen ficolin-1-rich granule lumen cytosol nucleus proteasome complex extracellular region nucleoplasm proteasome core complex
proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process
Cellular location:
cytoplasm extracellular exosome proteasome core complex, beta-subunit complex secretory granule lumen ficolin-1-rich granule lumen cytosol nucleus proteasome complex extracellular region nucleoplasm proteasome core complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 26 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
Proteasome subunit beta type-4
EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 3.4.25.1
Number of copies: 2
UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
proteasomal protein catabolic process negative regulation of inflammatory response to antigenic stimulus proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
lipopolysaccharide binding
Cellular location:
cytoplasm ciliary basal body nucleus proteasome complex proteasome core complex proteasome core complex, beta-subunit complex extracellular exosome cytosol mitochondrion nucleoplasm
proteasomal protein catabolic process negative regulation of inflammatory response to antigenic stimulus proteasome-mediated ubiquitin-dependent protein catabolic process
Molecular function:
lipopolysaccharide binding
Cellular location:
cytoplasm ciliary basal body nucleus proteasome complex proteasome core complex proteasome core complex, beta-subunit complex extracellular exosome cytosol mitochondrion nucleoplasm
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 29 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Homo sapiens | - | - | - |
ZINC ION
HET code: ZN
Number of copies: 1
ChEMBL ChEBI DrugBank
Name: HET code: ZN
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 65 Da | - |
ADENOSINE-5'-TRIPHOSPHATE
HET code: ATP
Number of copies: 4
ChEMBL ChEBI DrugBank
Name: HET code: ATP
Number of copies: 4
ChEMBL ChEBI DrugBank
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 507 Da | - |
MAGNESIUM ION
HET code: MG
Number of copies: 5
ChEBI DrugBank
Name: HET code: MG
Number of copies: 5
ChEBI DrugBank
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 24 Da | - |
ADENOSINE-5'-DIPHOSPHATE
HET code: ADP
Number of copies: 2
ChEMBL ChEBI DrugBank
Name: HET code: ADP
Number of copies: 2
ChEMBL ChEBI DrugBank
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 427 Da | - |