EMDB EMD-9041

Single particle reconstruction
3.55Å resolution

Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter captured by Corallopyronin

Map released:
Last modified:
Overview of EMD-9041
Source organism: Mycobacterium tuberculosis [1773]
Fitted atomic model: 6eec
Related EM entries by publication: EMD-9037, EMD-9039, EMD-9047
Primary publication:
Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Boyaci H, Chen J, Jansen R, Darst SA, Campbell EA
Nature - (2019)
PMID: 30626968

Function and Biology Details

Sample name: Mycobacterium tuberculosis RNAP open promoter complex
Ligands: methyl [(1E,5R)-5-{(3E)-3-[(2E,4E,8R,9E,12E)-1,8-dihydroxy-2,5,9-trimethyltetradeca-2,4,9,12-tetraen-1-ylidene]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hex-1-en-1-yl]carbamate, ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (65-MER), DNA (63-MER)
Proteins: Mycobacterium tuberculosis RNAP open promoter complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor SigA, RNA polymerase-binding protein RbpA, RNA polymerase-binding transcription factor CarD

Experimental Information Details

Resolution: 3.55Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)