EMD-9039
Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter
EMD-9039
Single-particle3.92 Å
Deposition: 13/08/2018
Map released: 21/11/2018
Last modified: 13/03/2024
Name: Mycobacterium tuberculosis RNAP promoter unwinding intermediate
Summary:
- Complex
- Protein
- DNA-directed RNA polymerase subunit alpha
- DNA-directed RNA polymerase subunit beta
- DNA-directed RNA polymerase subunit beta'
- DNA-directed RNA polymerase subunit omega
- RNA polymerase sigma factor SigA
- RNA polymerase-binding protein RbpA
- RNA polymerase-binding transcription factor CarD
- DNA
- Ligand
Mycobacterium tuberculosis RNAP promoter unwinding intermediate
Name: Natural source [1]
Organism: Mycobacterium tuberculosis
DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Number of copies: 2
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 2
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
protein dimerization activity DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex plasma membrane cytosol cytoplasm cell wall
DNA-templated transcription transcription, DNA-templated
Molecular function:
protein dimerization activity DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex plasma membrane cytosol cytoplasm cell wall
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 37 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
DNA-templated transcription response to antibiotic transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity ribonucleoside binding
Cellular location:
DNA-directed RNA polymerase complex peptidoglycan-based cell wall cell wall plasma membrane cytosol
DNA-templated transcription response to antibiotic transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity ribonucleoside binding
Cellular location:
DNA-directed RNA polymerase complex peptidoglycan-based cell wall cell wall plasma membrane cytosol
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 125 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 148 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
DNA-directed RNA polymerase subunit omega
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Name: EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [8]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex cell wall cytosol
DNA-templated transcription transcription, DNA-templated
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex cell wall cytosol
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 11 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
RNA polymerase sigma factor SigA
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [9]
Biological process:
DNA-templated transcription initiation response to water DNA-templated transcription, initiation
Molecular function:
DNA binding sigma factor activity
Cellular location:
peptidoglycan-based cell wall cytoplasm cell wall plasma membrane
DNA-templated transcription initiation response to water DNA-templated transcription, initiation
Molecular function:
DNA binding sigma factor activity
Cellular location:
peptidoglycan-based cell wall cytoplasm cell wall plasma membrane
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 58 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
RNA polymerase-binding protein RbpA
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
positive regulation of DNA-templated transcription positive regulation of transcription, DNA-templated
Molecular function:
nucleic acid binding bacterial-type RNA polymerase core enzyme binding bacterial-type RNA polymerase holo enzyme binding
Cellular location:
peptidoglycan-based cell wall cell wall
positive regulation of DNA-templated transcription positive regulation of transcription, DNA-templated
Molecular function:
nucleic acid binding bacterial-type RNA polymerase core enzyme binding bacterial-type RNA polymerase holo enzyme binding
Cellular location:
peptidoglycan-based cell wall cell wall
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 12 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
RNA polymerase-binding transcription factor CarD
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Name: Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [3]
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 17 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
DNA (65-MER)
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 27 kDa | - |
DNA (60-MER)
Number of copies: 1
Name: Number of copies: 1
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 27 kDa | - |