EMD-9039

Single-particle
3.92 Å
EMD-9039
3D View Gallery
Deposition: 13/08/2018
Map released: 21/11/2018
Last modified: 13/03/2024
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9039
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EMICSS annotations (xml)

Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter

EMD-9039

Single-particle
3.92 Å
EMD-9039
3D View Gallery
Deposition: 13/08/2018
Map released: 21/11/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Mycobacterium tuberculosis RNAP promoter unwinding intermediate
Complex [1]
Name: Mycobacterium tuberculosis RNAP promoter unwinding intermediate
Natural source [1]
Organism: Mycobacterium tuberculosis
Protein [7]
LEVO
Name: DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Number of copies: 2
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
protein dimerization activity DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex plasma membrane cytosol cytoplasm cell wall
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 37 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
DNA-templated transcription response to antibiotic transcription, DNA-templated
Molecular function:
DNA binding DNA-directed 5'-3' RNA polymerase activity ribonucleoside binding
Cellular location:
DNA-directed RNA polymerase complex peptidoglycan-based cell wall cell wall plasma membrane cytosol
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 125 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 148 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit omega
EC number: 2.7.7.6
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [8]
Biological process:
DNA-templated transcription transcription, DNA-templated
Molecular function:
DNA-directed 5'-3' RNA polymerase activity DNA binding
Cellular location:
peptidoglycan-based cell wall DNA-directed RNA polymerase complex cell wall cytosol
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 11 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: RNA polymerase sigma factor SigA
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [9]
Biological process:
DNA-templated transcription initiation response to water DNA-templated transcription, initiation
Molecular function:
DNA binding sigma factor activity
Cellular location:
peptidoglycan-based cell wall cytoplasm cell wall plasma membrane
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 58 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: RNA polymerase-binding protein RbpA
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
positive regulation of DNA-templated transcription positive regulation of transcription, DNA-templated
Molecular function:
nucleic acid binding bacterial-type RNA polymerase core enzyme binding bacterial-type RNA polymerase holo enzyme binding
Cellular location:
peptidoglycan-based cell wall cell wall
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 12 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: RNA polymerase-binding transcription factor CarD
Number of copies: 1
UniProtKB UniProtKB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [3]
Biological process:
rRNA transcription stringent response regulation of growth rate

Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 17 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
DNA [2]
DNA
Name: DNA (65-MER)
Number of copies: 1
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 27 kDa -
DNA
Name: DNA (60-MER)
Number of copies: 1
Natural source
Organism: Mycobacterium tuberculosis
Molecular weight
Experimental Theoretical Method
- 27 kDa -
Ligand [2]
Name: ZINC ION
HET code: ZN
Number of copies: 2
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -
Name: MAGNESIUM ION
HET code: MG
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -