EMDB EMD-9039

Single particle reconstruction
3.92Å resolution

Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter

Map released:
Last modified:
Overview of EMD-9039
Source organism: Mycobacterium tuberculosis [1773]
Fitted atomic model: 6ee8
Related EM entries by publication: EMD-9037, EMD-9041, EMD-9047
Primary publication:
Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Boyaci H, Chen J, Jansen R, Darst SA, Campbell EA
Nature - (2019)
PMID: 30626968

Function and Biology Details

Sample name: Mycobacterium tuberculosis RNAP promoter unwinding intermediate
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (65-MER), DNA (60-MER)
Proteins: Mycobacterium tuberculosis RNAP promoter unwinding intermediate, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor SigA, RNA polymerase-binding protein RbpA, RNA polymerase-binding transcription factor CarD

Experimental Information Details

Resolution: 3.92Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)