EMDB EMD-8997

Helical reconstruction
3.5Å resolution

13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules decorated with kinesin-1 motor domain

Map released:
Overview of EMD-8997
Source organism: Homo sapiens [9606]
Fitted atomic model: 6e7b
Related EM entry by publication: EMD-8998
Primary publication:
Human beta-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability.
Ti SC, Alushin GM, Kapoor TM
Dev. Cell - (2018)
PMID: 30245156

Function and Biology Details

Sample name: 13-pf 3-start GMPCPP-human alpha1B/beta3 microtubule decorated with kinesin-1 motor domain
Ligands: MAGNESIUM ION, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, GUANOSINE-5'-TRIPHOSPHATE
Proteins: 13-pf 3-start GMPCPP-human alpha1B/beta3 microtubule decorated with kinesin-1 motor domain, Tubulin beta-3 chain, Tubulin alpha-1B chain

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)