EMD-8782

Single-particle
3.37 Å
EMD-8782
3D View Gallery
Deposition: 21/06/2017
Map released: 19/07/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8782
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5w81 summary report (pdf.gz) Map and 5w81 FULL report (pdf.gz)
EMICSS annotations (xml)

Phosphorylated, ATP-bound structure of zebrafish cystic fibrosis transmembrane conductance regulator (CFTR)

EMD-8782

Single-particle
3.37 Å
EMD-8782
3D View Gallery
Deposition: 21/06/2017
Map released: 19/07/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5.5 mg/mL
Buffer
pH: 7.5
Grid
Mesh: 400
Model: Quantifoil R1.2/1.3
Material: GOLD
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 0.8 µm - 2.5 µm
Nominal magnification: 61199.0
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Dimensions: 7420 pixel x 7676 pixel
Frames per image: 1-50
Number of grids: 2
Number of real images: 7434
Average exposure time: 0.14 s
Average electron dose per image: 1.68 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.37 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 777102
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN 9.11 -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-8461
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
FREALIGN 9.11 -
Particle selection [1]
Selected Ref. model Method Software Details
900000 - - - -
Final 3D classification
Number of classes: 4
Software [1]
Name Version Details
RELION 2.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Map with B-factor sharpened
⬡ Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 0.82 Å 0.82 Å 0.82 Å
Contour list
Primary Level Source
True 1.9 AUTHOR