EMDB EMD-8749

Single particle reconstruction
3.9Å resolution

cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex

Map released:
Overview of EMD-8749
Source organisms:
Fitted atomic model: 5vzl
Primary publication:
Disabling Cas9 by an anti-CRISPR DNA mimic.
Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA
Sci Adv 3 e1701620-e1701620 (2017)
PMID: 28706995

Function and Biology Details

Sample name: the complex of Streptococcus progenies Cas9 with sgRNA and AcrIIA4
Nucleic acid: single guide RNA (116-MER)
Proteins: the complex of Streptococcus progenies Cas9 with sgRNA and AcrIIA4, Streptococcus pyogenes M1 GAS, unidentified phage, CRISPR-associated endonuclease Cas9, phage anti-CRISPR AcrIIA4

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)