EMDB EMD-8616

Single particle reconstruction
3.6Å resolution

70S ribosome bound with cognate ternary complex base-paired to A site codon (Structure II)

Map released:
Last modified:
Overview of EMD-8616
Source organism: Escherichia coli (strain K12) [83333]
Fitted atomic model: 5uyl
Related EM entries by publication: EMD-8615, EMD-8617, EMD-8618, EMD-8619, EMD-8620
Primary publication:
Ensemble cryo-EM elucidates the mechanism of translation fidelity.
Loveland AB, Demo G, Grigorieff N, Korostelev AA
Nature 546 113-117 (2017)
PMID: 28538735

Function and Biology Details

Sample name: 70S ribosome bound with cognate ternary complex not base-paired to A site codon (Structure I)
Ligands: N-FORMYLMETHIONINE, PHENYLALANINE, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
Nucleic acids: 16S ribosomal RNA, 23S ribosomal RNA, 5S ribosomal RNA, tRNAfMet, mRNA, tRNAPhe
Proteins: 70S ribosome bound with cognate ternary complex not base-paired to A site codon (Structure I), 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L31, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S21, 50S ribosomal protein L1, Elongation factor Tu 2

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)