Single particle reconstruction
7.8Å resolution

3D-reconstruction of O3-33 from a grid prepared with a single round of blotting

Map released:
Last modified:
Overview of EMD-8613
Source organism: unidentified [32644]
Related EM entry by publication: EMD-8614
Primary publication:
Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids.
Snijder J, Borst AJ, Dosey A, Walls AC, Burrell A, Reddy VS, Kollman JM, Veesler D
J. Struct. Biol. 198 38-42 (2017)
PMID: 28254381

Function and Biology Details

Sample name: O3-33
Proteins: O3-33, Computationally designed octahedral protein cage O3-33

Experimental Information Details

Resolution: 7.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: O
Reconstruction software: Relion
Microscope: FEI TECNAI F20
Detector: GATAN K2 (4k x 4k)