EMDB EMD-8479

Single particle reconstruction
4.4Å resolution

Cryo-EM map of the transcriptional Mediator

Map released:
Last modified:
Overview of EMD-8479
Source organism: Schizosaccharomyces pombe [4896]
Fitted atomic model: 5u0p
Related EM entry by publication: EMD-8480
Primary publication:
Mediator structure and rearrangements required for holoenzyme formation.
Tsai KL, Yu X, Gopalan S, Chao TC, Zhang Y, Florens L, Washburn MP, Murakami K, Conaway RC, Conaway JW, Asturias FJ
Nature 544 196-201 (2017)
PMID: 28241144

Function and Biology Details

Sample name: Mediator complex
Proteins: Mediator complex, Mediator complex subunit 14, Mediator complex subunit 6, Mediator complex subunit 8, Mediator complex subunit 17, Mediator complex subunit 18, Mediator complex subunit 20, Mediator complex subunit 11, Mediator complex subunit 22, Mediator complex subunit 4, Mediator complex subunit 7, Mediator complex subunit 21, Mediator complex subunit 31, Mediator complex subunit 9, Mediator complex subunit 27, Mediator complex subunit 19, Mediator complex subunit 10

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)