Single particle reconstruction
3.3Å resolution

CRISPR RNA-guided surveillance complex

Map released:
Last modified:
Overview of EMD-8478
Source organism: Thermobifida fusca (strain YX) [269800]
Fitted atomic model: 5u0a
Related EM entry by publication: EMD-8477
Primary publication:
Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.
Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A
Cell 170 48-60.e11 (2017)
PMID: 28666122

Function and Biology Details

Sample name: CRISPR RNA-guided surveillance complex
Nucleic acids: crRNA, Target Strand, Nontarget Strand
Proteins: CRISPR RNA-guided surveillance complex, CRISPR-associated protein, Cse3 family, CRISPR-associated protein, Cse1 family, CRISPR-associated protein, Cse4 family, Cse2, CRISPR-associated protein, Cas5e family

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 (4k x 4k)