EMDB EMD-8362

Single particle reconstruction
3.3Å resolution

Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis: 60S-Nmd3-Tif6-Lsg1 Complex

Map released:
Last modified:
Overview of EMD-8362
Fitted atomic model: 5t62
Related EM entry by publication: EMD-8368
Primary publication:
Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis.
Malyutin AG, Musalgaonkar S, Patchett S, Frank J, Johnson AW
EMBO J. 36 854-868 (2017)
PMID: 28179369

Function and Biology Details

Sample name: 60S in complex with Nmd3, Lsg1, and Tif6
Ligands: MAGNESIUM ION, POTASSIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, water
Nucleic acids: Saccharomyces cerevisiae S288c 35S pre-ribosomal RNA miscRNA, Saccharomyces cerevisiae strain HB_C_OMARUNUI_6 chromosome XII sequence, Saccharomyces cerevisiae culture-collection CBS:2888 large subunit ribosomal RNA gene, partial sequence
Proteins: 60S in complex with Nmd3, Lsg1, and Tif6, 60S-Nmd3-Tif6-Lsg1 complex with focus on Nmd3, Eukaryotic translation initiation factor 6, 60S ribosomal protein L2-A, 60S ribosomal protein L3, 60S ribosomal protein L4-A, 60S ribosomal protein L5, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, 60S ribosomal protein L9-A, 60S ribosomal protein L10, 60S ribosomal protein L11-A, 60S ribosomal protein L13-A, 60S ribosomal protein L14-A, 60S ribosomal protein L15-A, 60S ribosomal protein L16-A, 60S ribosomal protein L17-A, 60S ribosomal protein L18-A, 60S ribosomal protein L19-A, 60S ribosomal protein L20-A, 60S ribosomal protein L21-A, 60S ribosomal protein L22-A, 60S ribosomal protein L23-A, 60S ribosomal protein L24-A, 60S ribosomal protein L25, 60S ribosomal protein L26-A, 60S ribosomal protein L27-A, 60S ribosomal protein L28, 60S ribosomal protein L29, 60S ribosomal protein L30, 60S ribosomal protein L31-A, 60S ribosomal protein L32, 60S ribosomal protein L33-A, 60S ribosomal protein L34-A, 60S ribosomal protein L35-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A, 60S ribosomal protein L38, 60S ribosomal protein L39, Ubiquitin-60S ribosomal protein L40, 60S ribosomal protein L42-A, 60S ribosomal protein L43-A, Ribosomal Protein uL1, 60S ribosomal export protein NMD3, Large subunit GTPase 1

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscopes: FEI TECNAI F30, FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)