EMDB EMD-8014

Single particle reconstruction
3.6Å resolution

Body region of the U4/U6.U5 tri-snRNP

Map released:
Last modified:
Overview of EMD-8014
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5gap
Primary publication:
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K
Nature 530 298-302 (2016)
PMID: 26829225

Function and Biology Details

Sample name: Body region of the U4/U6.U5 tri-snRNP complex
Nucleic acids: U4 snRNA, 5' region, nucleotides 1-67, U6 snRNA, U5 snRNA
Proteins: Body region of the U4/U6.U5 tri-snRNP complex, unknown protein, Pre-mRNA-splicing factor 8, U4/U6 small nuclear ribonucleoprotein PRP4, Pre-mRNA-splicing factor 6, Spliceosomal protein DIB1, Pre-mRNA-processing factor 31, U4/U6 small nuclear ribonucleoprotein PRP3, 13 kDa ribonucleoprotein-associated protein, Pre-mRNA-splicing helicase BRR2

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)