EMDB EMD-8013

Single particle reconstruction
4.2Å resolution

Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP

Map released:
Last modified:
Overview of EMD-8013
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5gao
Primary publication:
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K
Nature 530 298-302 (2016)
PMID: 26829225

Function and Biology Details

Sample name: Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP
Nucleic acid: Saccharomyces cerevisiae strain UOA_M2 chromosome 5 sequence
Proteins: Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Snu66, Pre-mRNA-splicing helicase BRR2, Pre-mRNA-splicing factor 8

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)