EMDB EMD-8012

Single particle reconstruction
3.7Å resolution

The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom

Map released:
Last modified:
Overview of EMD-8012
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5gan
Primary publication:
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K
Nature 530 298-302 (2016)
PMID: 26829225

Function and Biology Details

Sample name: The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP
Ligand: GUANOSINE-5'-TRIPHOSPHATE
Nucleic acids: U4 snRNA, U6 snRNA, U5 snRNA
Proteins: The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP, Pre-mRNA-splicing factor 8, U4/U6 small nuclear ribonucleoprotein PRP4, Pre-mRNA-splicing factor 6, Spliceosomal protein DIB1, Pre-mRNA-processing factor 31, U4/U6 small nuclear ribonucleoprotein PRP3, Pre-mRNA-splicing helicase BRR2, Unknown protein, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Snu66, 13 kDa ribonucleoprotein-associated protein, U6 snRNA-associated Sm-like protein LSm2, U6 snRNA-associated Sm-like protein LSm3, U6 snRNA-associated Sm-like protein LSm4, U6 snRNA-associated Sm-like protein LSm5, U6 snRNA-associated Sm-like protein LSm6, U6 snRNA-associated Sm-like protein LSm7, U6 snRNA-associated Sm-like protein LSm8, Pre-mRNA-splicing factor SNU114

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)