EMDB EMD-8011

Single particle reconstruction
3.7Å resolution

Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP

Map released:
Last modified:
Overview of EMD-8011
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5gam
Primary publication:
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Angstrom resolution
Nguyen THD, Galej WP, Bai XC, Oubridge C, Scheres SHW, Newman AJ, Nagai K
Nature 530 298-302 (2016)
PMID: 26829225

Function and Biology Details

Sample name: Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP
Ligand: GUANOSINE-5'-TRIPHOSPHATE
Nucleic acids: U5 snRNA, U6 snRNA, 5' end
Proteins: Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP, Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor SNU114, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Unknown polypeptide, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)