EMD-7919

Single-particle
23.0 Å
EMD-7919
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Deposition: 24/05/2018
Map released: 27/06/2018
Last modified: 22/08/2018
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7919
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

VIC157 Fab in complex with Ebola virus GP

EMD-7919

Single-particle
23.0 Å
EMD-7919
3D View Gallery
Deposition: 24/05/2018
Map released: 27/06/2018
Last modified: 22/08/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.02 mg/mL
Details: This sample was monodisperse.
Buffer
pH: 7.4
Buffer components [2]:
Name Formula Concentration ChEBI
Sodium chlordie NaCl 150.0 mM
Tris(hydroxymethyl)aminomethane Tris 20.0 mM
Details: Solution was made from 10X concentration.
Staining
Type: NEGATIVE
Material: Uranyl formate
Details: Stained using 2% UF on carbon coated grids.
Grid
Mesh: 400
Model: Homemade
Material: COPPER
Pretreatment
Type: PLASMA CLEANING
Atmosphere: OTHER
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Microscopy [1]
Microscope: FEI TECNAI SPIRIT
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
C2 aperture diameter: 100.0 µm
Nominal CS: 2.2 mm
Nominal defocus: 1.5 µm - 0.001 µm
Nominal magnification: 52000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 4096 pixel x 4096 pixel
Number of grids: 1
Number of real images: 73
Average exposure time: 0.7 s
Average electron dose per image: 30.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 23.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 15745
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
EMAN2 2.1 -
Startup model [1]
Type: OTHER
Details:Common lines model.
⦨ Initial angle assignment
Type: COMMON LINE
Software [1]
Name Version Details
EMAN2 - -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
EMAN2 - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: VIC157 antibody Fab in complex with Ebola virus GP
⬡ Geometry
X Y Z
Dimensions 80 80 80
Origin 0 0 0
Spacing 80 80 80
Voxel size 4.1 Å 4.1 Å 4.1 Å
Contour list
Primary Level Source
True 26.5 AUTHOR