EMD-7492
2.20 A MicroED structure of proteinase K at 4.3 e- / A^2
EMD-7492
Electron Crystallography2.2 Å
Deposition: 02/03/2018
Map released: 16/05/2018
Last modified: 04/10/2023
Concentration: 25
mg/mL
Buffer
pH: 8.0
Buffer components [2]:
Buffer components [2]:
Name | Formula | Concentration | ChEBI |
---|---|---|---|
Ammonium sulfate | N2H8SO4 | 1.2 M | |
Tris | C4H11NO3 | 0.1 M |
Vitrification
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 1200 mm
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 1200 mm
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 289
Number of diffraction images: 289
Average exposure time: 5.1 s
Average electron dose per image: 0.0357 e/Å2
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 289
Number of diffraction images: 289
Average exposure time: 5.1 s
Average electron dose per image: 0.0357 e/Å2
Final
reconstruction
Molecular replacement
Software
[1]
Name | Version | Details |
---|---|---|
MOLREP | 11.4.05 | - |
⌯ Symmetry determination
Software [1]
Name | Version | Details |
---|---|---|
POINTLESS | 1.11.3 | - |
Merging
Software [1]
Name | Version | Details |
---|---|---|
AIMLESS | 0.5.32 | - |
Crystallography statistics
Number of intensities measured:
65656
Number of structure factors: 11559
Fourier space coverage: 94.3
R sym: 0.567
R merge: 0.567
Overall phase error: 51.81
Overall phase residual: 51.81
Phase error rejection criteria: 0
High resolution: 2.2 Å
Number of structure factors: 11559
Fourier space coverage: 94.3
R sym: 0.567
R merge: 0.567
Overall phase error: 51.81
Overall phase residual: 51.81
Phase error rejection criteria: 0
High resolution: 2.2 Å
Shell list
[1]
High resolution | Low resolution | Number of structure factors | Phase residual | Fourier space coverage | Multiplicity |
---|---|---|---|---|---|
2.2 Å | 2.26 Å | 834 | 62.35 | 94.54 | 5.8 |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 2.20 A MicroED density map of proteinase K at 4.3 e- / A^2
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 2.20 A MicroED density map of proteinase K at 4.3 e- / A^2
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 79 | 72 | 72 |
Origin | -64 | -6 | -37 |
Spacing | 96 | 96 | 136 |
Voxel size | 0.70054793 Å | 0.70054793 Å | 0.7421507 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.218655 | AUTHOR |