EMD-7485

Single-particle
21.0 Å
EMD-7485
3D View Gallery
Deposition: 27/02/2018
Map released: 11/04/2018
Last modified: 16/11/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7485
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cas4-dependent prespacer processing ensures high-fidelity programming of CRISPR arrays

EMD-7485

Single-particle
21.0 Å
EMD-7485
3D View Gallery
Deposition: 27/02/2018
Map released: 11/04/2018
Last modified: 16/11/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Staining
Type: NEGATIVE
Material: Uranyl acetate
Grid
Details: Unspecified
Pretreatment
Type: GLOW DISCHARGE
Atmosphere: AIR
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Microscopy [1]
Microscope: JEOL 2010
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Average electron dose per image: 30.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 21.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 4000
Algorithm: BACK PROJECTION
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: OTHER
Details:Ab initio
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
CryoSPARC - -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cas1-Cas4 complex
⬡ Geometry
X Y Z
Dimensions 64 64 64
Origin 0 0 0
Spacing 64 64 64
Voxel size 3.6 Å 3.6 Å 3.6 Å
Contour list
Primary Level Source
True 0.055 EMDB