EMD-7442

Single-particle
2.8 Å
EMD-7442
3D View Gallery
Deposition: 31/01/2018
Map released: 11/04/2018
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7442
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6caj summary report (pdf.gz) Map and 6caj FULL report (pdf.gz)
EMICSS annotations (xml)

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B from Homo sapiens

EMD-7442

Single-particle
2.8 Å
EMD-7442
3D View Gallery
Deposition: 31/01/2018
Map released: 11/04/2018
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 1.63 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.8 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 202125
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
FREALIGN - -
Startup model [1]
Type: NONE
Details:Initial model generated using cryoSPARC ab-initio modeling
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
FREALIGN - -
Particle selection [1]
Selected Ref. model Method Software Details
202125 - - - -
CTF correction
Software [1]
Name Version Details
Gctf 1.06 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Primary
⬡ Geometry
X Y Z
Dimensions 458 458 458
Origin 0 0 0
Spacing 458 458 458
Voxel size 0.83799994 Å 0.83799994 Å 0.83799994 Å
Contour list
Primary Level Source
True 4.62 AUTHOR