EMD-7324

Single-particle
3.7 Å
EMD-7324 Deposition: 29/12/2017
Map released: 14/03/2018
Last modified: 08/01/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7324

Yeast nucleolar pre-60S ribosomal subunit (state 2)

EMD-7324

Single-particle
3.7 Å
EMD-7324 Deposition: 29/12/2017
Map released: 14/03/2018
Last modified: 08/01/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.6
Grid
Mesh: 400
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere: AIR
Support Film [1]
Material Topology Thickness
CARBON LACEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 3.5 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 1.56 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 201114
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2.1 -
Initial angle assignment
Final angle assignment
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: State 2 map
Geometry
X Y Z
Dimensions 480 480 480
Origin 0 0 0
Spacing 480 480 480
Voxel size 1.3 Å 1.3 Å 1.3 Å
Contour list
Primary Level Source
True 0.02 AUTHOR