EMD-7324
Yeast nucleolar pre-60S ribosomal subunit (state 2)
EMD-7324
Single-particle3.7 Å
Deposition: 29/12/2017
Map released: 14/03/2018
Last modified: 08/01/2020
Buffer
pH: 7.6
Grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: FEI VITROBOT MARK IV
Chamber humidity: 100%
Chamber temperature: 295 K
Instrument: FEI VITROBOT MARK IV
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 3.5 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 3.5 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 1.56 e/Å2
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 1.56 e/Å2
Final
reconstruction
Resolution: 3.7
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 201114
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 201114
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 2.1 | - |
⦨ Initial angle
assignment
Type:
PROJECTION MATCHING
⦩ Final angle assignment
Type:
PROJECTION MATCHING
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 4.1 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: State 2 map
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: State 2 map
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 480 | 480 | 480 |
Origin | 0 | 0 | 0 |
Spacing | 480 | 480 | 480 |
Voxel size | 1.3 Å | 1.3 Å | 1.3 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.02 | AUTHOR |