EMDB EMD-7324

Single particle reconstruction
3.7Å resolution

Yeast nucleolar pre-60S ribosomal subunit (state 2)

Map released:
Last modified:
Overview of EMD-7324
Source organism: Saccharomyces cerevisiae BY4741 [1247190]
Fitted atomic model: 6c0f
Related EM entry by publication: EMD-7445
Primary publication:
Modular assembly of the nucleolar pre-60S ribosomal subunit.
Sanghai ZA, Miller L, Molloy KR, Barandun J, Hunziker M, Chaker-Margot M, Wang J, Chait BT, Klinge S
Nature 556 126-129 (2018)
PMID: 29512650

Function and Biology Details

Sample name: Yeast large subunit processome
Ligand: ZINC ION
Nucleic acids: Saccharomyces cerevisiae S288c 35S pre-ribosomal RNA miscRNA, 5.8S rRNA, ITS2
Proteins: Yeast large subunit processome, Ribosome biogenesis protein NSA1, 60S ribosomal protein L3, 60S ribosomal protein L4-A, Protein MAK16, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, Ribosome production factor 1, Proteasome-interacting protein CIC1, 60S ribosomal protein L13-A, 60S ribosomal protein L14-A, 60S ribosomal protein L15-A, 60S ribosomal protein L16-A, 60S ribosomal protein L17-A, 60S ribosomal protein L18-A, 60S ribosomal protein L20-A, 60S ribosomal protein L23-A, Nucleolar GTP-binding protein 1, 60S ribosomal protein L26-A, Nucleolar protein 16, Ribosomal RNA-processing protein 14, Ribosome biogenesis protein BRX1, 60S ribosomal protein L32, 60S ribosomal protein L33-A, 60S ribosomal protein L35-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A, Brx1-associated peptide, rRNA-processing protein EBP2, Pescadillo homolog, Ribosome biogenesis protein 15, ATP-dependent RNA helicase HAS1, Protein MAK11, Ribosome biogenesis protein ERB1, Ribosome biogenesis protein RLP7, Ribosome biogenesis protein RLP24, Ribosome biogenesis protein SSF1, Ribosomal RNA-processing protein 15, Eukaryotic translation initiation factor 6, Ribosomal RNA-processing protein 1

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)