EMD-7119

Single-particle
3.6 Å
EMD-7119
3D View Gallery
Deposition: 18/11/2017
Map released: 05/12/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7119
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6bo5 summary report (pdf.gz) Map and 6bo5 FULL report (pdf.gz)
EMICSS annotations (xml)

TRPV2 ion channel in partially closed state

EMD-7119

Single-particle
3.6 Å
EMD-7119
3D View Gallery
Deposition: 18/11/2017
Map released: 05/12/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: .5 mg/mL
Buffer
pH: 8.0
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Chamber humidity: 98%
Chamber temperature: 293 K
Microscopy [1]
Microscope: JEOL 3200FSC
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 63.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 50509
⌯ Applied Symmetry
Point group: C4
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: NONE
⦨ Initial angle assignment
Type: RANDOM ASSIGNMENT
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 1.4 -
Particle selection [1]
Selected Ref. model Method Software Details
160100 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: TRPV2 ion channel in a partially closed state and with a deletion in the pore turret domain.
⬡ Geometry
X Y Z
Dimensions 220 220 220
Origin 0 0 0
Spacing 220 220 220
Voxel size 1.23 Å 1.23 Å 1.23 Å
Contour list
Primary Level Source
True 0.018 AUTHOR