EMDB EMD-7109

Single particle reconstruction
3.3Å resolution

S. cerevisiae spliceosomal post-catalytic P complex

Map released:
Last modified:
Overview of EMD-7109
Source organisms:
Fitted atomic model: 6bk8
Primary publication:
Structure of the yeast spliceosomal postcatalytic P complex.
Liu S, Li X, Zhang L, Jiang J, Hill RC, Cui Y, Hansen KC, Zhou ZH, Zhao R
Science 358 1278-1283 (2017)
PMID: 29146870

Function and Biology Details

Sample name: Spliceosomal P complex
Ligands: MAGNESIUM ION, INOSITOL HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, ZINC ION
Nucleic acids: U2 snRNA, U5 snRNA, U6 snRNA, RNA (34-MER), RNA (59-MER)
Proteins: Spliceosomal P complex, Pre-mRNA-splicing factor Prp8, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor BUD31, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor CWC22, Pre-mRNA-processing factor Prp17, Pre-mRNA-splicing factor Prp18, Pre-mRNA-splicing factor SLU7, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, Pre-mRNA-splicing factor SYF2, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor CLF1, Pre-mRNA-splicing factor SYF1, Unknown protein fragment, Unknown protein fragment, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein Sm D1, Lea1, Msl1, Pre-mRNA-processing factor Prp19, Pre-mRNA-splicing factor SNT309

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)