EMD-7097 Experiments and Validation

Doubly PafE-capped 20S core particle in Mycobacterium tuberculosis

Single particle reconstruction
Overview of EMD-7097
Sample name: Doubly PafE-capped 20S CP
Organism: Mycobacterium tuberculosis
Fitted atomic model: 6bgl
Related EM entry by publication: EMD-7098

Map parameters

Recommended contour level: 0.007 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.09 × 1.09 × 1.09 Å
Minimum density: -0.012
Maximum density: 0.031
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Doubly PafE-capped 20S CP
Proteins: Doubly PafE-capped 20S CP, Proteasome subunit alpha, Bacterial proteasome activator, Proteasome subunit beta

Validation information

Experimental information

 

Image processing

Applied symmetry: D7
Number of particles: 51091

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 1.8999999999999999
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE