EMD-7068
rsCSP + Fab311 Complex
EMD-7068
Single-particle25.0 Å
Deposition: 09/10/2017
Map released: 15/11/2017
Last modified: 13/12/2017
Concentration: 0.01
mg/mL
Buffer
pH: 7.4
Buffer components [2]:
Buffer components [2]:
Name | Formula | Concentration | ChEBI |
---|---|---|---|
Sodium chloride | NaCl | 150.0 mM | |
Tris | (HOCH2)3CNH2 | 50.0 mM |
Staining
Type:
NEGATIVE
Material: 2% (w/v) Uranyl Formate
Details: Add 3 uL sample, blot, add 3 uL 2% uranyl formate, blot immediately, add 3 uL 2% uranyl formate, blot after 30 seconds
Material: 2% (w/v) Uranyl Formate
Details: Add 3 uL sample, blot, add 3 uL 2% uranyl formate, blot immediately, add 3 uL 2% uranyl formate, blot after 30 seconds
Grid
Mesh: 400
Model: Homemade
Material: COPPER
Details: 15 mA
Model: Homemade
Material: COPPER
Details: 15 mA
Pretreatment
Support Film [1]
Material | Topology | Thickness |
---|---|---|
CELLULOSE ACETATE | CONTINUOUS | - |
Microscope: FEI TECNAI SPIRIT
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.5 µm -
Nominal magnification: 52000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.5 µm -
Nominal magnification: 52000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
TVIPS TEMCAM-F416 (4k x 4k)
Number of grids: 1
Average electron dose per image: 25.0 e/Å2
Number of grids: 1
Average electron dose per image: 25.0 e/Å2
Final
reconstruction
Resolution: 25.0
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6320
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6320
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 2.0 | - |
⦨ Initial angle
assignment
Type:
PROJECTION MATCHING
Number of reference projections: 20
Details: Used EMAN2 e2initialmodel
Number of reference projections: 20
Details: Used EMAN2 e2initialmodel
Software
[1]
Name | Version | Details |
---|---|---|
EMAN | 2 | E2initialmodel.py |
⦩ Final angle assignment
Type:
NOT APPLICABLE
Particle selection
[1]
Selected | Ref. model | Method | Software | Details |
---|---|---|---|---|
6320 | - | - | - | - |
Final 3D classification
Number of classes:
3
Avg. number of members per classes: 7000.0
Avg. number of members per classes: 7000.0
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 2.0 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: RsCSP + Fab311 Complex
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: RsCSP + Fab311 Complex
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 160 | 160 | 160 |
Origin | 0 | 0 | 0 |
Spacing | 160 | 160 | 160 |
Voxel size | 2.05 Å | 2.05 Å | 2.05 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.0228 | AUTHOR |