EMD-7068

Single-particle
25.0 Å
EMD-7068 Deposition: 09/10/2017
Map released: 15/11/2017
Last modified: 13/12/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7068

rsCSP + Fab311 Complex

EMD-7068

Single-particle
25.0 Å
EMD-7068 Deposition: 09/10/2017
Map released: 15/11/2017
Last modified: 13/12/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 7.4
Buffer components [2]:
Name Formula Concentration ChEBI
Sodium chloride NaCl 150.0 mM
Tris (HOCH2)3CNH2 50.0 mM
Staining
Type: NEGATIVE
Material: 2% (w/v) Uranyl Formate
Details: Add 3 uL sample, blot, add 3 uL 2% uranyl formate, blot immediately, add 3 uL 2% uranyl formate, blot after 30 seconds
Grid
Mesh: 400
Model: Homemade
Material: COPPER
Details: 15 mA
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CELLULOSE ACETATE CONTINUOUS -
Microscopy [1]
Microscope: FEI TECNAI SPIRIT
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.5 µm -
Nominal magnification: 52000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Number of grids: 1
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6320
Software [1]
Name Version Details
RELION 2.0 -
Initial angle assignment
Type: PROJECTION MATCHING
Number of reference projections: 20
Details: Used EMAN2 e2initialmodel
Software [1]
Name Version Details
EMAN 2 E2initialmodel.py
Final angle assignment
Particle selection [1]
Selected Ref. model Method Software Details
6320 - - - -
Final 3D classification
Number of classes: 3
Avg. number of members per classes: 7000.0
Software [1]
Name Version Details
RELION 2.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: RsCSP + Fab311 Complex
Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 2.05 Å 2.05 Å 2.05 Å
Contour list
Primary Level Source
True 0.0228 AUTHOR