EMD-7066
Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11 heavy chain and FAB H11 light chain
EMD-7066
Single-particle6.5 Å
Deposition: 05/10/2017
Map released: 10/01/2018
Last modified: 28/04/2021
Name: Insulin degrading enzyme
Summary:
Insulin degrading enzyme
Details: Cryo-EM structure of human Apo insulin degrading enzyme
Name: Details: Cryo-EM structure of human Apo insulin degrading enzyme
Natural source [1]
Organism: Homo sapiens
Recombinant Expression [1]
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL | - | - |
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
100 kDa | - | - |
Insulin-degrading enzyme
EC number: 3.4.24.56
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: EC number: 3.4.24.56
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [40]
Biological process:
proteolysis involved in protein catabolic process peptide catabolic process antigen processing and presentation of endogenous peptide antigen via MHC class I regulation of aerobic respiration insulin catabolic process insulin metabolic process determination of adult lifespan amyloid-beta clearance cellular protein catabolic process proteolysis ubiquitin recycling protein catabolic process amyloid-beta metabolic process bradykinin catabolic process proteolysis involved in cellular protein catabolic process positive regulation of protein catabolic process amyloid-beta clearance by cellular catabolic process insulin receptor signaling pathway hormone catabolic process positive regulation of protein binding
Molecular function:
virus receptor activity protein homodimerization activity insulin binding peptide binding zinc ion binding ATP binding ubiquitin-dependent protein binding metalloendopeptidase activity endopeptidase activity
Cellular location:
external side of plasma membrane peroxisome peroxisomal matrix cytosol extracellular exosome nucleus cytoplasm extracellular space cell surface mitochondrion basolateral plasma membrane
proteolysis involved in protein catabolic process peptide catabolic process antigen processing and presentation of endogenous peptide antigen via MHC class I regulation of aerobic respiration insulin catabolic process insulin metabolic process determination of adult lifespan amyloid-beta clearance cellular protein catabolic process proteolysis ubiquitin recycling protein catabolic process amyloid-beta metabolic process bradykinin catabolic process proteolysis involved in cellular protein catabolic process positive regulation of protein catabolic process amyloid-beta clearance by cellular catabolic process insulin receptor signaling pathway hormone catabolic process positive regulation of protein binding
Molecular function:
virus receptor activity protein homodimerization activity insulin binding peptide binding zinc ion binding ATP binding ubiquitin-dependent protein binding metalloendopeptidase activity endopeptidase activity
Cellular location:
external side of plasma membrane peroxisome peroxisomal matrix cytosol extracellular exosome nucleus cytoplasm extracellular space cell surface mitochondrion basolateral plasma membrane
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 111 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
FAB H11 heavy chain
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Name: Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 23 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |
FAB H11 light chain
Number of copies: 2
Name: Number of copies: 2
Natural source
Organism: Homo sapiens
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 23 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | - | - | - |