EMDB EMD-7059

Single particle reconstruction
3.9Å resolution

The cryo-EM structure of a bacterial class I transcription activation complex

Map released:
Last modified:
Overview of EMD-7059
Source organism: Escherichia coli [562]
Fitted atomic model: 6b6h
Related EM entry by publication: EMD-7060
Primary publication:
Structural basis of bacterial transcription activation.
Liu B, Hong C, Huang RK, Yu Z, Steitz TA
Science 358 947-951 (2017)
PMID: 29146813

Function and Biology Details

Sample name: The complex of class-I bacterial transcription activation complex
Cellular component: The complex of class-I bacterial transcription activation complex
Ligands: ZINC ION, MAGNESIUM ION, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Nucleic acids: SYNTHETIC NONTEMPLATE STRAND DNA (88-MER), SYNTHETIC TEMPLATE STRAND DNA (88-MER), NASCENT RNA 3-MER
Proteins: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD, cAMP-activated global transcriptional regulator CRP, DNA-directed RNA polymerase subunit alpha

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)