EMDB EMD-7024

Single particle reconstruction
2.7Å resolution

Cryo-EM structure of the small subunit of Leishmania ribosome bound to paromomycin

Map released:
Last modified:
Overview of EMD-7024
Source organisms:
Fitted atomic model: 6az1
Related EM entry by publication: EMD-7025
Primary publication:
Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin.
Shalev-Benami M, Zhang Y, Rozenberg H, Nobe Y, Taoka M, Matzov D, Zimmerman E, Bashan A, Isobe T, Jaffe CL, Yonath A, Skiniotis G
Nat Commun 8 1589-1589 (2017)
PMID: 29150609

Function and Biology Details

Sample name: Leishmania donovani 91S ribosome SSU
Ligands: MAGNESIUM ION, PAROMOMYCIN, water
Nucleic acids: ribosomal RNA 18S, tRNA-Phe, P-site tRNA, E-site tRNA, mRNA
Proteins: Leishmania donovani 91S ribosome SSU, Ribosomal protein s1e, ribosomal protein S2, ribosomal protein S3, ribosomal protein S4, ribosomal protein S4e, ribosomal protein S5, ribosomal protein S6e, ribosomal protein S7, ribosomal protein S7e, ribosomal protein S8, ribosomal protein S8e, ribosomal protein S9, ribosomal protein S10, ribosomal protein S10e, ribosomal protein S11, ribosomal protein S12, ribosomal protein S12e, ribosomal protein S13, ribosomal protein S14, ribosomal protein S15, ribosomal protein S17, ribosomal protein S17e, ribosomal protein S19, ribosomal protein S19e, ribosomal protein S21e, ribosomal protein S24e, ribosomal protein S25e, ribosomal protein S26e, ribosomal protein S27e, ribosomal protein S28e, ribosomal protein S30e, ribosomal protein S31e, LACK1

Experimental Information Details

Resolution: 2.7Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)