EMD-6958

Tomography
43.7 Å
EMD-6958
Gallery
Deposition: 02/05/2018
Map released: 23/05/2018
Last modified: 12/12/2018
Overview Sample Experiment Validation Volume Browser Additional data Links
Overview Sample Experiment Validation Volume Browser Additional data Links

EMD-6958
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Doublet microtubule of zebrafish sperm axoneme, twister_null mutant

EMD-6958

Tomography
43.7 Å
EMD-6958
Gallery
Deposition: 02/05/2018
Map released: 23/05/2018
Last modified: 12/12/2018
Overview Sample Experiment Validation Volume Browser Additional data Links
Method: Tomography
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.2
Grid
Mesh: 300
Model: Homemade
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Instrument: LEICA EM GP
Fiducial Markers
Manufacturer Fiducial type Diameter
BBInternational - 15 nanometer
Aurion - 15 nanometer
Sectioning
Details: NO SECTIONING
Microscopy [1]
Microscope: JEOL 3100FFC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal magnification: 30000.0
Specimen holder model: GATAN 914 HIGH TILT LIQUID NITROGEN CRYO TRANSFER TOMOGRAPHY HOLDER
Cooling holder cryogen: NITROGEN
Specialist optics
Energy filter
Name: In-column Omega Filter
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 4096 pixel x 4096 pixel
Average electron dose per image: 1.6 e/Å2
Image processing [1]
Final reconstruction
Resolution: 43.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 60
Algorithm: BACK PROJECTION
Software [1]
Name Version Details
IMOD - -
CTF correction
Software [1]
Name Version Details
IMOD - -
Tomogram
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Doublet microtubule structure from zebrafish sperm flagella, twister_null mutant
⬡ Geometry
X Y Z
Dimensions 120 180 120
Origin 0 0 0
Spacing 120 180 120
Voxel size 7.2 Å 7.2 Å 7.2 Å
Contour list
Primary Level Source
True - AUTHOR