EMDB EMD-6878

Single particle reconstruction
3.65Å resolution

Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)

Map released:
Overview of EMD-6878
Source organisms:
Fitted atomic model: 5z3g
Primary publication:
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.
Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K
Protein Cell - (2018)
PMID: 29557065

Function and Biology Details

Sample name: Rpf1-TAP pre-60S
Nucleic acids: 25S rRNA, 5.8S rRNA, ITS2 RNA
Proteins: Rpf1-TAP pre-60S, Ribosome biogenesis protein RLP7, Ribosome biogenesis protein 15, 60S ribosomal protein L3, 60S ribosomal protein L4-A, Proteasome-interacting protein CIC1, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, 60S ribosomal protein L9-A, Nucleolar protein 7, Eukaryotic translation initiation factor 6, Ribosome production factor 1, 60S ribosomal protein L13-A, 60S ribosomal protein L14-A, 60S ribosomal protein L15-A, 60S ribosomal protein L16-A, 60S ribosomal protein L17-A, 60S ribosomal protein L18-A, Ribosome biogenesis protein NSA1, 60S ribosomal protein L20-A, Ribosomal RNA-processing protein 1, ATP-dependent RNA helicase HAS1, Protein MAK16, Ribosome biogenesis protein BRX1, rRNA-processing protein EBP2, 60S ribosomal protein L26-A, Unassigned, 60S ribosomal protein L32, 60S ribosomal protein L33-A, 60S ribosomal protein L35-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A

Experimental Information Details

Resolution: 3.65Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER