EMD-6804

Single-particle
12.8 Å
EMD-6804
Deposition: 03/08/2017
Map released: 08/08/2018
Obsolete date: 14/11/2018
Last modified: 12/12/2018
Overview

EMD-6804

 This entry has been made obsolete.

Cryo-EM reconstruction of the INO80 complex

EMD-6804

Single-particle
12.8 Å
EMD-6804
Deposition: 03/08/2017
Map released: 08/08/2018
Obsolete date: 14/11/2018
Last modified: 12/12/2018
Method: Single-particle
Aggregation State: Cell
Specimen preparation [1]
Buffer
pH: 7.5
Staining
Type: NEGATIVE
Material: Uranyl Formate
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 36.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 12.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 23176
Algorithm: FOURIER SPACE
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the INO80 complex
⬡ Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 1.42 Å 1.42 Å 1.42 Å
Contour list
Primary Level Source
True 0.0233 AUTHOR